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This assay differentiates between previous exposure/infection and current infection. Serological testing is not recommended for diagnosis. Per current recommendations, culture of lower respiratory secretions and/or the Legionella urinary test should be used for diagnosis of infection with Legionella.
Expected Turnaround Time
1 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
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Specimen Requirements
Specimen
Serum
Volume
0.4 mL
Minimum Volume
0.2 mL
Container
Red-top tube or gel-barrier tube
Storage Instructions
Room temperature
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 7 days |
Refrigerated | 7 days |
Frozen | 7 days |
Freeze/thaw cycles | Stable x3 |
Causes for Rejection
Hemolysis; lipemia; gross bacterial contamination
Test Details
Use
This assay can be used to identify exposure to Legionella pneumophila but serological testing is not recommended for diagnosis of Legionella infection. This assay does not differentiate between previous exposure/infection and current infection. Per current recommendations, culture of lower respiratory secretions and/or the Legionella urinary antigen test should be used for diagnosis of infection with Legionella.
Limitations
This assay detects antibodies against L. pneumophila serogroups 1 through 6. It does not detect non-pneumophila, or atypical, Legionella species. This assay cannot differentiate between previous exposure/infection and current infection. A diagnosis should be made on the basis of Legionella serology results alone; test results should be interpreted in conjunction with clinical evaluation and other diagnostic procedures.
Methodology
Enzyme-linked immunosorbent assay (ELISA)
Reference Interval
Non-reactive
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 164616 | Legionella pneumophila Abs. | 7947-5 | 164618 | Legionella pneumophila Abs. | 7947-5 |
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The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf