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Antibody titers for mycelial and yeast antigens of Histoplasma
Expected Turnaround Time
2 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Serum
Volume
0.6 mL
Minimum Volume
0.25 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Storage Instructions
Refrigerate
Stability Requirements
Temperature | Period |
|---|---|
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x2 |
Causes for Rejection
Hemolysis; lipemia; gross bacterial contamination
Test Details
Use
Immunodiffusion test is used to detect antibodies to Histoplasma capsulatum. The antigen used is a culture filtrate. Histoplasmin H and M preciptins can be identified by the assay. The M band often appears first and may occur without the H band. M preciptin is found in about 70% of both acute and chronic histoplasmosis cases. Both M and H occur together in only about 10% of patients. Results should be interpreted in the context of clinical presentation and other laboratory findings (e.g., fungal culture).
Limitations
Negative fungal serology does not rule out the possibility of current infection.
Methodology
Double immunodiffusion (DID)
Reference Interval
Negative: <1:1
References
Heiner DC. Diagnosis of histoplasmosis using precipitin reactions in agargel. Pediatrics. 1958 Oct;22(4 Part 1):616-627.13590840
Palmer D, Kaufman L, Kaplan W, Cavallaro J. Serodiagnosis of Mycotic Diseases. Charles C Thomas, Springfield, IL; 1977.
Reiss E, Kauffman L, Kovacs J, Lindsley M. Clinical Immunomycology, p.559-583. In N. Rose, R. Hamilton, and B. Detrick (ed.), Manual of Clinical Laboratory Immunology. ASM Press, Washington D.C.; 2002.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 164319 | Histoplasma Abs, Qn, DID | 27266-6 | 164320 | Histoplasma Abs, Qn, DID | 27266-6 |
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