Test Details
Methodology
Chemiluminescent microparticle immunoassay (CMIA); line blot (immunoblot)
Result Turnaround Time
1 - 4 days
Use
This test is used to screen patients for infection with human T-lymphotropic virus and, if positive, confirm infection with either HTLV-1 or HTLV-2.
Specimen Requirements
Limitations
HTLV-1 or HTLV-2 immunoblot testing should only be performed on patient specimens previously found positive for HTLV infection by immunoassay (EIA).
Specimen
Serum or plasma
Volume
2 mL
Minimum Volume
1.5 mL
Container
Red-top tube, gel-barrier tube, or lavender-top (EDTA) tube
Storage Instructions
Refrigerate.
Causes for Rejection
Gross bacterial contamination; hemolysis; lipemia
Collection Instructions
Centrifuge sample to separate serum or plasma. For non-gel-barrier tubes, transfer serum or plasma to a plastic transport tube.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 7 days |
Refrigerated | 14 days |
Frozen | 6 months |
Freeze/thaw cycles | Stable x6 |
Note: Stability provided by manufacturer or literature reference.
Reference Range
Negative
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 164277 | HTLV-I/II Antibodies, Qual. | 29901-6 | 164278 | HTLV-I/II Antibodies, Qual | 29901-6 | |
| Order Code | 164277 | |||||
| Order Code Name | HTLV-I/II Antibodies, Qual. | |||||
| Order Loinc | 29901-6 | |||||
| Result Code | 164278 | |||||
| Result Code Name | HTLV-I/II Antibodies, Qual | |||||
| UofM | ||||||
| Result LOINC | 29901-6 |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 164133 | HTLV-I/II Immunoblot | 164130 | HTLV-I IB | 6432-9 | |
| Reflex 1 | ||||||
| Order Code | 164133 | |||||
| Order Name | HTLV-I/II Immunoblot | |||||
| Result Code | 164130 | |||||
| Result Name | HTLV-I IB | |||||
| UofM | ||||||
| Result LOINC | 6432-9 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 164133 | HTLV-I/II Immunoblot | 164132 | HTLV-II IB | 26656-9 | |
| Reflex 1 | ||||||
| Order Code | 164133 | |||||
| Order Name | HTLV-I/II Immunoblot | |||||
| Result Code | 164132 | |||||
| Result Name | HTLV-II IB | |||||
| UofM | ||||||
| Result LOINC | 26656-9 | |||||