Find Locations
For hours, walk-ins and appointments.Synonyms
- BP180 and BP230 IgG
- Pemphigoid Autoantibodies
Expected Turnaround Time
5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Serum
Volume
2 mL
Minimum Volume
1 mL (Note: This volume does not allow for repeat testing.)
Container
Gel-barrier tube, red-top tube or serum transfer tube
Collection
Serum samples may be stored for up to eight hours at room temperature before freezing at -20°C. Samples should not be repeatedly frozen and thawed.
Storage Instructions
Frozen at -20°C
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | Unstable (stability provided by manufacturer or literature reference) |
Refrigerated | Unstable (stability provided by manufacturer or literature reference) |
Frozen | 14 days (stability provided by manufacturer or literature reference) |
Freeze/thaw cycles | Stable x3 (stability provided by manufacturer or literature reference) |
Causes for Rejection
Bacterial contamination, hemolysis, lipemia
Test Details
Use
This test is a preferred screening test for patients suspected to have bullous pemphigoid and its variants. The antibody levels can be used to monitor the effectiveness of drug treatment.
The diverse spectrum of pemphigoid diseases is characterized by the formation of subepidermal blisters, which can develop in both the skin and mucous membranes. These conditions involve circulating autoantibodies that specifically target components found in the dermal-epidermal junction. Bullous pemphigoid (BP) presents with tense, swollen blisters that can appear on either inflamed or non-inflamed skin, with mucous membranes being rarely affected. The patient's serum contains IgG antibodies that primarily target hemidesmosomal proteins, namely BP180 and BP230. BP180 is a transmembrane glycoprotein with its primary immunogenic epitopes located in the extracellular 16th non-collagenous domain (BP180-NC16 A).
Pemphigoid gestationis is a variant of BP that occurs in pregnant women and during the postpartum period, resulting in the development of urticarial plaques and/or tense blisters. Mucous membrane pemphigoid affects one or more mucous membranes such as the oral, ocular, genital and anal regions, and can also involve the skin. Patients with this condition exhibit low-titer IgG/IgA autoantibodies directed against components of the basement membrane zone, with BP180 and laminin 332 being the two primary targets.
Methodology
Enzyme Immunoassay (EIA)
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 163995 | BP180 and BP230 IgG | 92671-7 | 163996 | BP180 IgG | RU/mL | 53842-1 |
| 163995 | BP180 and BP230 IgG | 92671-7 | 163997 | BP230 IgG | RU/mL | 53843-9 |
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