Lyme Disease, Line Blot

TEST: 163600
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CPT: 86617(x2)
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Synonyms

  • Borrelia burgdorferi Antibodies

Test Includes

Line blot analysis and interpretations for IgG- and IgM-specific antibodies

Special Instructions

This test is intended as the second step in testing serum samples found to have been equivocal or positive using an ELISA or IFA test as the first step to provide supportive evidence of infection with B burgdorferi. For CSF, order test 160457.

Expected Turnaround Time

1 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Specimen Requirements

Specimen

Serum

Volume

1 mL

Minimum Volume

0.5 mL

Container

Red-top tube or gel-barrier tube or serum transfer tube

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination

Test Details

Use

Detect antibodies specific for B burgdorferi. This test is intended as the second step in testing serum samples found to have been equivocal or positive using an ELISA or IFA test as the first step to provide supportive evidence of infection with B burgdorferi.

Methodology

Line blot

Reference Interval

Negative

Additional Information

Provides both IgM and IgG Line blot results. Increased specificity reduces cross-reactivity from other spirochetes. Detect and analyze antibodies against specific B burgdorferi proteins. The sensitivity of a Line blot assay by itself is not sufficient to assess Lyme infection adequately. Both EIA and Line blot tests should be performed.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
163600 Lyme, Line Blot, Serum 60342-3 163635 IgG P93 Ab. 9597-6
163600 Lyme, Line Blot, Serum 60342-3 163605 IgG P66 Ab. 9596-8
163600 Lyme, Line Blot, Serum 60342-3 163623 IgG P58 Ab. 9595-0
163600 Lyme, Line Blot, Serum 60342-3 163641 IgG P45 Ab. 9594-3
163600 Lyme, Line Blot, Serum 60342-3 163607 IgG P41 Ab. 9593-5
163600 Lyme, Line Blot, Serum 60342-3 163598 IgG P39 Ab. 9592-7
163600 Lyme, Line Blot, Serum 60342-3 163633 IgG P30 Ab. 9591-9
163600 Lyme, Line Blot, Serum 60342-3 163636 IgG P28 Ab. 9590-1
163600 Lyme, Line Blot, Serum 60342-3 163642 IgG P23 Ab. 9589-3
163600 Lyme, Line Blot, Serum 60342-3 163627 IgG P18 Ab. 9588-5
163600 Lyme, Line Blot, Serum 60342-3 163640 Lyme IgG Line Blot Interp. 13502-0
163600 Lyme, Line Blot, Serum 60342-3 163616 IgM P41 Ab. 9587-7
163600 Lyme, Line Blot, Serum 60342-3 163599 IgM P39 Ab. 9599-2
163600 Lyme, Line Blot, Serum 60342-3 163638 IgM P23 Ab. 9598-4
163600 Lyme, Line Blot, Serum 60342-3 163639 Lyme IgM Line Blot Interp. 13503-8

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf