Test Details
Methodology
Enzyme-linked immunosorbent assay (ELISA)
Result Turnaround Time
1 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
Establish evidence of infection/exposure to Bordetella pertussis, the causative agent of whooping cough
Custom Additional Information
Patients with acute infection develop IgG, IgM, and IgA antibodies to fimbrial agglutinogens, and IgM and IgA antibodies are probably diagnostic. Following vaccination, IgG and IgM antibodies can be demonstrated. IgG antibodies can only be used to diagnose acute infection when paired sera are available and a rise in antibody level can be demonstrated.
Specimen Requirements
Specimen
Serum
Volume
0.4 mL
Minimum Volume
0.2 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x4 |
Reference Range
• Negative: <0.95 index
• Equivocal: 0.95−1.04 index
• Positive: >1.04 index
Storage Instructions
Room temperature
Causes for Rejection
Hemolysis; lipemia; gross bacterial contamination
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 161745 | B pertussis IgG Ab | 42330-1 | 161751 | B pertussis IgG Ab | index | 42330-1 |
| Order Code | 161745 | |||||
| Order Code Name | B pertussis IgG Ab | |||||
| Order Loinc | 42330-1 | |||||
| Result Code | 161751 | |||||
| Result Code Name | B pertussis IgG Ab | |||||
| UofM | index | |||||
| Result LOINC | 42330-1 |