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Viral Hepatitis Screening and Diagnosis (HAV, HBV, HCV)

Test Number 144445
Test number copied
CPT 86704; 86706; 86708; 86803; 87340
Synonyms
  • Hepatitis
  • Hepatitis A
  • Hepatitis B
  • Hepatitis C

Test Details

Methodology

Immunochemiluminometric assay (ICMA)

Result Turnaround Time

1 - 2 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

Hepatitis A Virus (HAV) Antibody, Total with reflex to IgM [144226]; Hepatitis B Virus (HBV) Screening and Diagnosis [144473]; Hepatitis C Virus (HCV) Antibody with reflex to Quantitative Real-time PCR [144050]

Use

Aid in the diagnosis of viral hepatitis due to infection with HAV, HBV, and/or HCV.

Special Instructions

If reflex test is performed, additional charges/CPT code(s) may apply.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Limitations

The assays in this panel have not been FDA cleared or approved for the screening of blood or plasma donors. Assay performance characteristics have not been established for immunocompromised or immunosuppressed patients, cord blood, or patients less than 2 years of age.

References

Abara WE, Oaseem A, Schillie S. McMahon BJ, Harris AM, High Value Care Task Force of the American College of Physicians and the Centers for Disease Control and Prevention. Hepatitis B Vaccination, Screening, and Linkage to Care: Best Practice Advice from the American College of Physicians and the Centers for Disease Control and Prevention. Ann Intern Med. 2017 Dec 5;167(11):794-804.29159414
American Association for the Study of Liver Diseases, Infectious Diseases Society of America. HCV Guidance: Recommendations for Testing, Managing, and Treating Hepatitis C: HCV Testing and Linkage to Care. HCVGuidelines.org web site: https://www.hcvguidelines.org/evaluate/testing-and-linkage. Accessed June 2021.
Centers for Disease Control and Prevention. Hepatitis B. CDC web site: www.cdc.gov/hepatitis/hbv/index.htm. Accessed May 2021.
Terrault NA, Lok ASF, McMahon BJ, et al. Update on Prevention, Diagnosis, and Treatment of Chronic Hepatitis B: AASLD 2018 Hepatitis B Guidance. Hepatology. 2018 Apr;67(4):1560-1599.29405329
Workowski KA, Bolan GA; Centers for Disease Control and Prevention. Sexually transmitted diseases treatment guidelines, 2015. MMWR Recomm Rep. 2015 Jun 5;64(RR-03):1-137.26042815

Custom Additional Information

HAV Serology Interpretation: Key - Analyte present: + Analyte absent: - Test not indicated: TNI

Interpretation

HAV Ab, Total

HAV Ab, IgM

Susceptible/no indication of infection

-

TNI

Previously vaccinated against or infected with HAV

+

-

Acute infection

+

+

HBV Serology Interpretation: Key - Analyte present: + Analyte absent: - Test not indicated: TNI

Interpretation

HBsAg

anti-HBs

anti-HBc

IgM anti-HBc

* Multiple possibilities: resolved infection (most common); false-positive anti-HBc (susceptible); "low-level" chronic infection; resolving acute infection.

HCV Serology Interpretation: Key - Analyte present: + Analyte absent: - Test not indicated: TNI

Interpretation

HCV Ab

HCV RNA, NAA

*For persons who might have been exposed to HCV within the past 6 months, testing for HCV RNA or follow-up testing for HCV antibody should be performed. For persons who are immunocompromised, testing for HCV RNA should be performed.

**Repeat HCV-RNA testing if the person tested is suspected to have had HCV exposure within the past 6 months or has clinical evidence of HCV disease.

No HCV antibody detected, no indication of infection*

-

TNI

HCV antibody detected but no evidence of current infection**

+

-

HCV antibody and viral RNA detected consistent with current infection

+

+

Susceptible/no evidence of infection

-

-

-

TNI

Immune due to natural resolved infection

-

+

+

TNI

Immune due to vaccination

-

+

-

TNI

Acute infection

+

-

+

+

Chronic infection

+

-

+

-

Interpretation unclear*

-

-

+

±

Specimen Requirements

Specimen

Serum or EDTA plasma

Volume

7 mL

Minimum Volume

3.5 mL

Container

Gel-barrier tube, plasma preparation tube (PPT), serum transfer tube, EDTA-plasma transfer tube

Collection Instructions

Centrifuge sample within 24 hours of collection. If tube other than a gel-barrier tube is used, or if sample will be shipped frozen, transfer separated serum/plasma to a screw-cap polypropylene transport tube.

Storage Instructions

Refrigerated (preferred)

HAV and HBV: stable 14 days at room temperature, refrigerated (preferred), and frozen

HCV: stable 24 hours at room temperature, 6 days refrigerated or 6 weeks frozen

Causes for Rejection

Heat-inactivated specimens; cord blood; cadaveric specimens; or body fluids other than serum or EDTA plasma; gross hemolysis; excessive lipemia; improper labeling; frozen gel-barrier tube

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
144445 HAV, HBV, HCV 24363-4 006726 Hep A Ab, Total 13951-9
144445 HAV, HBV, HCV 24363-4 006510 HBsAg Screen 5196-1
144445 HAV, HBV, HCV 24363-4 006408 Hep B Surface Ab, Qual 22322-2
144445 HAV, HBV, HCV 24363-4 006718 Hep B Core Ab, Tot 13952-7
144445 HAV, HBV, HCV 24363-4 006719 Rfx to HBc IgM 77202-0
144445 HAV, HBV, HCV 24363-4 006720 Interpretation 56850-1
144445 HAV, HBV, HCV 24363-4 144035 HCV Ab 40726-2
Order Code144445
Order Code NameHAV, HBV, HCV
Order Loinc24363-4
Result Code006726
Result Code NameHep A Ab, Total
UofM
Result LOINC13951-9
Order Code144445
Order Code NameHAV, HBV, HCV
Order Loinc24363-4
Result Code006510
Result Code NameHBsAg Screen
UofM
Result LOINC5196-1
Order Code144445
Order Code NameHAV, HBV, HCV
Order Loinc24363-4
Result Code006408
Result Code NameHep B Surface Ab, Qual
UofM
Result LOINC22322-2
Order Code144445
Order Code NameHAV, HBV, HCV
Order Loinc24363-4
Result Code006718
Result Code NameHep B Core Ab, Tot
UofM
Result LOINC13952-7
Order Code144445
Order Code NameHAV, HBV, HCV
Order Loinc24363-4
Result Code006719
Result Code NameRfx to HBc IgM
UofM
Result LOINC77202-0
Order Code144445
Order Code NameHAV, HBV, HCV
Order Loinc24363-4
Result Code006720
Result Code NameInterpretation
UofM
Result LOINC56850-1
Order Code144445
Order Code NameHAV, HBV, HCV
Order Loinc24363-4
Result Code144035
Result Code NameHCV Ab
UofM
Result LOINC40726-2
Reflex Table for Hep A Ab, Total
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 006734 Hep A Ab, IgM 006734 Hep A Ab, IgM 13950-1
Reflex 1
Order Code006734
Order NameHep A Ab, IgM
Result Code006734
Result NameHep A Ab, IgM
UofM
Result LOINC13950-1
Reflex Table for HBsAg Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 016105 HBsAg Confirmation 016105 HBsAg Confirmation 7905-3
Reflex 1
Order Code016105
Order NameHBsAg Confirmation
Result Code016105
Result NameHBsAg Confirmation
UofM
Result LOINC7905-3
Reflex Table for Rfx to HBc IgM
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 016881 Hep B Core Ab, IgM 016881 Hep B Core Ab, IgM 24113-3
Reflex 1
Order Code016881
Order NameHep B Core Ab, IgM
Result Code016881
Result NameHep B Core Ab, IgM
UofM
Result LOINC24113-3
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 144030 Interpretation: 144030 Interpretation: 13955-0
Reflex 1
Order Code144030
Order NameInterpretation:
Result Code144030
Result NameInterpretation:
UofM
Result LOINC13955-0
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550362 HCV RT-PCR, Quant (Non-Graph) 550338 Hepatitis C Quantitation IU/mL 11011-4
Reflex 1
Order Code550362
Order NameHCV RT-PCR, Quant (Non-Graph)
Result Code550338
Result NameHepatitis C Quantitation
UofMIU/mL
Result LOINC11011-4
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550362 HCV RT-PCR, Quant (Non-Graph) 550338 Hepatitis C Quantitation IU/mL 11011-4
Reflex 1
Order Code550362
Order NameHCV RT-PCR, Quant (Non-Graph)
Result Code550338
Result NameHepatitis C Quantitation
UofMIU/mL
Result LOINC11011-4
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550362 HCV RT-PCR, Quant (Non-Graph) 550339 HCV log10 log10 IU/mL 38180-6
Reflex 1
Order Code550362
Order NameHCV RT-PCR, Quant (Non-Graph)
Result Code550339
Result NameHCV log10
UofMlog10 IU/mL
Result LOINC38180-6
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550362 HCV RT-PCR, Quant (Non-Graph) 550314 Test Information: 19147-8
Reflex 1
Order Code550362
Order NameHCV RT-PCR, Quant (Non-Graph)
Result Code550314
Result NameTest Information:
UofM
Result LOINC19147-8
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550362 HCV RT-PCR, Quant (Non-Graph) 550396 Interpretation: 62365-2
Reflex 1
Order Code550362
Order NameHCV RT-PCR, Quant (Non-Graph)
Result Code550396
Result NameInterpretation:
UofM
Result LOINC62365-2