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Viral Hepatitis Screening and Diagnosis (HBV, HCV)

Test Number 144025
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CPT

86704; 86706; 86803; 87340
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  • Updated on 04/08/2025

Test Details

Methodology

Immunochemiluminometric assay (ICMA) (see individual tests for methodologies)

Result Turnaround Time

1 - 3 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

Hepatitis B Surface Antigen (HBsAg) Screen, Qualitative [006510]; Hepatitis B Surface Antibody, Qualitative [006395]; Hepatitis B Core Antibody, Total with reflex to IgM [160101]; Hepatitis C Virus (HCV) Antibody with reflex to Quantitative Real-time PCR [144050]

Use

This test is used to aid in the diagnosis of viral hepatitis due to infection with HBV and/or HCV.

Aid in the diagnosis of viral hepatitis due to infection with HBV and/or HCV.

This test is used to aid in the diagnosis of viral hepatitis due to infection with HBV and/or HCV.

Special Instructions

If reflex test is performed, additional charges/CPT code(s) may apply.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Limitations

The assays in this panel have not been FDA cleared or approved for the screening of blood or plasma donors. Assay performance characteristics have not been established for immunocompromised or immunosuppressed patients, cord blood, or patients less than 2 years of age.

Custom Additional Information

HBV Serology Interpretation: Key - Analyte present: + Analyte absent: - Test not indicated: TNI
InterpretationHBsAganti-HBsanti-HBcIgM anti-HBc
* Multiple possibilities: resolved infection (most common); false-positive anti-HBc (susceptible); "low-level" chronic infection; resolving acute infection.
Susceptible/no evidence of infection---TNI
Immune due to natural resolved infection-++TNI
Immune due to vaccination-+-TNI
Acute infection+-++
Chronic infection+-+-
Interpretation unclear*--+±
HCV Serology Interpretation: Key - Analyte present: + Analyte absent: - Test not indicated: TNI
InterpretationHCV AbHCV RNA, NAA

*For persons who might have been exposed to HCV within the past 6 months, testing for HCV RNA or follow-up testing for HCV antibody should be performed. For persons who are immunocompromised, testing for HCV RNA should be performed.

**Repeat HCV-RNA testing if the person tested is suspected to have had HCV exposure within the past 6 months or has clinical evidence of HCV disease.

No HCV antibody detected, no indication of infection*-TNI
HCV antibody detected but no evidence of current infection**+-
HCV antibody and viral RNA detected consistent with current infection++

Specimen Requirements

Specimen

Serum or EDTA plasma

Volume

4 mL

Minimum Volume

2 mL (Note: This volume does not allow for repeat testing.)

2 mL

2 mL (Note: This volume does not allow for repeat testing.)

Container

Gel-barrier tube, plasma preparation tube (PPT), serum transfer tube, EDTA-plasma transfer tube

Collection Instructions

Centrifuge sample within 24 hours of collection. If tube other than a gel-barrier tube is used, or if sample will be shipped frozen, transfer separated serum/plasma to a screw-cap polypropylene transport tube.

Ship refrigerated (preferred) or frozen.

Storage Instructions

Refrigerated (preferred)

HBV: stable 14 days at room temperature, refrigerated (preferred), and frozen

HCV: stable 24 hours at room temperature, 6 days refrigerated or 6 weeks frozen

Causes for Rejection

Heat-inactivated specimens; cord blood; cadaveric specimens; or body fluids other than serum or EDTA plasma; gross hemolysis; excessive lipemia; improper labeling; frozen gel-barrier tube

References

Abara WE, Oaseem A, Schillie S. McMahon BJ, Harris AM, High Value Care Task Force of the American College of Physicians and the Centers for Disease Control and Prevention. Hepatitis B Vaccination, Screening, and Linkage to Care: Best Practice Advice from the American College of Physicians and the Centers for Disease Control and Prevention. Ann Intern Med. 2017 Dec 5;167(11):794-804.29159414
American Association for the Study of Liver Diseases, Infectious Diseases Society of America. HCV Guidance: Recommendations for Testing, Managing, and Treating Hepatitis C: HCV Testing and Linkage to Care. HCVGuidelines.org web site: https://www.hcvguidelines.org/evaluate/testing-and-linkage. Accessed June 2021.
Centers for Disease Control and Prevention. Hepatitis B. CDC web site: www.cdc.gov/hepatitis/hbv/index.htm. Accessed May 2021.
Terrault NA, Lok ASF, McMahon BJ, et al. Update on Prevention, Diagnosis, and Treatment of Chronic Hepatitis B: AASLD 2018 Hepatitis B Guidance. Hepatology. 2018 Apr;67(4):1560-1599.29405329
Workowski KA, Bolan GA; Centers for Disease Control and Prevention. Sexually transmitted diseases treatment guidelines, 2015. MMWR Recomm Rep. 2015 Jun 5;64(RR-03):1-137.26042815

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
144025 Viral Hepatitis HBV, HCV 92889-5 006510 HBsAg Screen 5196-1
144025 Viral Hepatitis HBV, HCV 92889-5 006408 Hep B Surface Ab, Qual 22322-2
144025 Viral Hepatitis HBV, HCV 92889-5 006718 Hep B Core Ab, Tot 13952-7
144025 Viral Hepatitis HBV, HCV 92889-5 144035 HCV Ab 40726-2
Order Code144025
Order Code NameViral Hepatitis HBV, HCV
Order Loinc92889-5
Result Code006510
Result Code NameHBsAg Screen
UofM
Result LOINC5196-1
Order Code144025
Order Code NameViral Hepatitis HBV, HCV
Order Loinc92889-5
Result Code006408
Result Code NameHep B Surface Ab, Qual
UofM
Result LOINC22322-2
Order Code144025
Order Code NameViral Hepatitis HBV, HCV
Order Loinc92889-5
Result Code006718
Result Code NameHep B Core Ab, Tot
UofM
Result LOINC13952-7
Order Code144025
Order Code NameViral Hepatitis HBV, HCV
Order Loinc92889-5
Result Code144035
Result Code NameHCV Ab
UofM
Result LOINC40726-2
Reflex Table for HBsAg Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 016105 HBsAg Confirmation 016105 HBsAg Confirmation 7905-3
Reflex 1
Order Code016105
Order NameHBsAg Confirmation
Result Code016105
Result NameHBsAg Confirmation
UofM
Result LOINC7905-3
Reflex Table for Hep B Core Ab, Tot
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 126881 HBcIgM 016881 Hep B Core Ab, IgM 24113-3
Reflex 1
Order Code126881
Order NameHBcIgM
Result Code016881
Result NameHep B Core Ab, IgM
UofM
Result LOINC24113-3
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 144030 Interpretation: 144030 Interpretation: 13955-0
Reflex 1
Order Code144030
Order NameInterpretation:
Result Code144030
Result NameInterpretation:
UofM
Result LOINC13955-0
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550362 HCV RT-PCR, Quant (Non-Graph) 550338 Hepatitis C Quantitation IU/mL 11011-4
Reflex 1
Order Code550362
Order NameHCV RT-PCR, Quant (Non-Graph)
Result Code550338
Result NameHepatitis C Quantitation
UofMIU/mL
Result LOINC11011-4
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550362 HCV RT-PCR, Quant (Non-Graph) 550338 Hepatitis C Quantitation IU/mL 11011-4
Reflex 1
Order Code550362
Order NameHCV RT-PCR, Quant (Non-Graph)
Result Code550338
Result NameHepatitis C Quantitation
UofMIU/mL
Result LOINC11011-4
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550362 HCV RT-PCR, Quant (Non-Graph) 550339 HCV log10 log10 IU/mL 38180-6
Reflex 1
Order Code550362
Order NameHCV RT-PCR, Quant (Non-Graph)
Result Code550339
Result NameHCV log10
UofMlog10 IU/mL
Result LOINC38180-6
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550362 HCV RT-PCR, Quant (Non-Graph) 550314 Test Information: 19147-8
Reflex 1
Order Code550362
Order NameHCV RT-PCR, Quant (Non-Graph)
Result Code550314
Result NameTest Information:
UofM
Result LOINC19147-8
Reflex Table for HCV Ab
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550362 HCV RT-PCR, Quant (Non-Graph) 550396 Interpretation: 62365-2
Reflex 1
Order Code550362
Order NameHCV RT-PCR, Quant (Non-Graph)
Result Code550396
Result NameInterpretation:
UofM
Result LOINC62365-2