Test Details
Methodology
Polymerase chain reaction (PCR)
Result Turnaround Time
3 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
Vesicular specimens collected from persons infected with a non-variola Orthopoxvirus (such as vaccinia, mpox, or cowpox) are expected to produce a positive result with this assay. Although this assay does not differentiate vaccinia or mpox virus from cowpox, camelpox, ectromelia or gerbilpox virus, a positive result with this assay in the United States is most likely due to mpox virus or vaccinia virus. However, potential exposure to other Orthopoxviruses should be considered.
Limitations
A false negative result may occur if a sample is improperly collected, transported or handled. False negative results may occur if the quantity of viral DNA present in the sample is below the limit of detection for the assay. An inconclusive result may be returned if the lesion is not adequately sampled.
Custom Additional Information
If mpox is suspected, do not submit specimens for any Viral Cultures. Testing for HSV and VZV by molecular methods is available. See Herpes Simplex Virus (HSV) and Varicella Zoster Virus (VZV) Profile (Lesions), PCR [139367] for further information.
Specimen Requirements
Specimen
Lesion swab
Volume
One swab in UTM or VTM
Minimum Volume
One swab in UTM or VTM
Container
Universal Transport Media (UTM) or Viral Transport Media (VTM)
Collection Instructions
Vigorously swab or brush the base of the lesion with a sterile dry polyester, rayon or Dacron swab. Insert the swab into the tube containing UTM or VTM. Carefully break the swab at the scoreline and tightly close the sample. Some UTM kits may contain two swabs; however, only one swab needs to be collected and submitted for testing. If multiple lesions with differing appearances are present, consider submitting an additional UTM/VTM collection, as described above, for each lesion.
Stability Requirements
Temperature | Period |
|---|---|
Refrigerated | 7 days (stability provided by manufacturer or literature reference) |
Frozen | 30 days (stability provided by manufacturer or literature reference) |
Storage Instructions
Refrigerate.
Causes for Rejection
Improperly submitted specimens, including grossly leaking samples; swabs submitted in non-validated transport media, including 0.9% saline or liquid Amies (E-Swabs); swab specimens submitted in aerobic or anaerobic transport devices
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 140230 | Monkeypox (Orthopoxvirus), PCR | 100434-0 | 140231 | Orthopoxvirus DNA | 100434-0 | |
| Order Code | 140230 | |||||
| Order Code Name | Monkeypox (Orthopoxvirus), PCR | |||||
| Order Loinc | 100434-0 | |||||
| Result Code | 140231 | |||||
| Result Code Name | Orthopoxvirus DNA | |||||
| UofM | ||||||
| Result LOINC | 100434-0 |