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Testosterone, Free, Direct With Total Testosterone

CPT

84402; 84403

Synonyms
  • Free Testosterone, Direct and Total

Test Details

Methodology

Free: Direct analog enzyme immunoassay (EIA); Total: electrochemiluminescence immunoassay (ECLIA)

Result Turnaround Time

4 - 7 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Use

Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states where the testosterone binding proteins may be altered (obesity, cirrhosis, thyroid disorders); see individual test descriptions for more information

Special Instructions

State patient's age and sex on the test request form.

This panel includes Testosterone, Total, which may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Limitations

A pediatric reference interval has not been established for the direct free testosterone test.

Specimen Requirements

Specimen

Serum

Volume

2 mL

Minimum Volume

1 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Collection Instructions

If a red-top tube is used, transfer separated serum to a plastic transport tube.

Stability Requirements

TemperaturePeriod
Room temperature14 days
Refrigerated14 days
Frozen14 days
Freeze/thaw cyclesStable x3

Storage Instructions

Room temperature

Causes for Rejection

Gross lipemia (may yield erroneously high free testosterone results); plasma specimen; gross hemolysis and/or gross icterus cause for rejection of direct free testosterone

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
140103 Testosterone,Free and Total 58952-3 004227 Testosterone ng/dL 2986-8
140103 Testosterone,Free and Total 58952-3 144981 Free Testosterone(Direct) pg/mL 2991-8
Order Code140103
Order Code NameTestosterone,Free and Total
Order Loinc58952-3
Result Code004227
Result Code NameTestosterone
UofMng/dL
Result LOINC2986-8
Order Code140103
Order Code NameTestosterone,Free and Total
Order Loinc58952-3
Result Code144981
Result Code NameFree Testosterone(Direct)
UofMpg/mL
Result LOINC2991-8