Test Details
Methodology
RT-PCR
Result Turnaround Time
2 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test is intended for use as an aid in the diagnosis of HIV-1 or HIV-2 infection, including acute or primary infection. Presence of HIV-1 or HIV-2 RNA in the plasma of patients without antibodies to HIV-1 or HIV-2 is indicative of acute or primary HIV-1 or HIV-2 infection. This assay may also be used as an additional test, when it is reactive, to confirm HIV-1 infection in an individual whose specimen is repeatedly reactive for HIV-1 and/or HIV-2 antibodies. This assay is not intended for use in donor testing.
Specimen Requirements
Specimen
Plasma or serum
Volume
1 mL
Minimum Volume
650 µL (Note: This volume does not allow for repeat testing.)
650 µL |
650 µL (Note: This volume does not allow for repeat testing.) |
Container
Lavender-top (EDTA) tube, BD SST tube, BD Vacutainer PPT plasma preparation tube
Collection Instructions
Collect specimen in lavender-top (EDTA), gel-barrier (SST tube) or a PPT plasma preparation tube. Specimens centrifugation within 24 hours of collection. Transfer serum or plasma to a polypropylene screw-cap tube for shipment.
Collect specimen in lavender-top (EDTA), gel-barrier (SST tube) or a PPT plasma preparation tube. Specimens centrifugation within 24 hours of collection. Transfer serum or plasma to a polypropylene screw-cap tube for shipment. Ship frozen. |
Collect specimen in lavender-top (EDTA), gel-barrier (SST tube) or a PPT plasma preparation tube. Specimens centrifugation within 24 hours of collection. Transfer serum or plasma to a polypropylene screw-cap tube for shipment.
|
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 6 weeks |
| Freeze/thaw cycles | Stable x3 |
Note: Stability provided by manufacturer or literature reference.
Storage Instructions
Refrigerated or frozen
|
Refrigerated or frozen |
Causes for Rejection
Specimen grossly hemolyzed; specimen received at room temperature
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 139825 | HIV-1/HIV-2 Qualitative RNA | 96557-4 | 139826 | HIV-1 RNA | 25835-0 | |
| 139825 | HIV-1/HIV-2 Qualitative RNA | 96557-4 | 139827 | HIV-2 RNA | 69353-1 | |
| Order Code | 139825 | |||||
| Order Code Name | HIV-1/HIV-2 Qualitative RNA | |||||
| Order Loinc | 96557-4 | |||||
| Result Code | 139826 | |||||
| Result Code Name | HIV-1 RNA | |||||
| UofM | ||||||
| Result LOINC | 25835-0 | |||||
| Order Code | 139825 | |||||
| Order Code Name | HIV-1/HIV-2 Qualitative RNA | |||||
| Order Loinc | 96557-4 | |||||
| Result Code | 139827 | |||||
| Result Code Name | HIV-2 RNA | |||||
| UofM | ||||||
| Result LOINC | 69353-1 |