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Herpes Simplex Virus (HSV) Types 1/2, DNA PCR

CPT 87529(x2)
Synonyms
  • HSV Types 1 and 2, DNA PCR (Real Time)

Test Details

Methodology

Real-time polymerase chain reaction (PCR)

Result Turnaround Time

2 - 4 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

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Use

This test is intended for use as an aid in the diagnosis of herpes simplex virus (HSV) infections; it also differentiates HSV-1 from HSV-2. PCR testing of blood, serum, or plasma samples is clinically useful only in potential cases of disseminated HSV infection (neonates, immunosuppressed individuals) and not as an aid in the diagnosis of either mucosal or CNS disease.

Special Instructions

HSV PCR is a single test that both detects the presence of HSV DNA and determines which type is present in the positive samples. There is no mechanism, therefore, for testing for HSV-1 without simultaneously testing for HSV-2.

Specimen Requirements

Specimen

Swab, whole blood, serum, or plasma

Volume

One swab; or 1 mL whole blood, serum, or plasma

Minimum Volume

One swab; or 0.5 mL whole blood, serum, or plasma

Container

Universal transport medium (swab), red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or yellow-top (ACD) tube

Collection Instructions

To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Whole blood is stable at room temperature or refrigerated for seven days; do not freeze. Swab samples are stable at room temperature, refrigerated, or frozen for seven days. Serum or plasma samples are stable at room temperature or refrigerated for seven days or frozen for 90 days.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
138651 HSV 1/2 PCR 87431-3 139332 HSV-1 DNA 16130-7
138651 HSV 1/2 PCR 87431-3 139333 HSV-2 DNA 16131-5
Order Code138651
Order Code NameHSV 1/2 PCR
Order Loinc87431-3
Result Code139332
Result Code NameHSV-1 DNA
UofM
Result LOINC16130-7
Order Code138651
Order Code NameHSV 1/2 PCR
Order Loinc87431-3
Result Code139333
Result Code NameHSV-2 DNA
UofM
Result LOINC16131-5