Test Details
Methodology
Latex enhanced immunoturbidimetric method
Result Turnaround Time
2 - 4 days
Use
Elevated levels of plasma MPO are a sensitive indicator of inflammatory disorders.
Specimen Requirements
Limitations
Erratic results can be observed if plasma is not centrifuged and separated from red cells immediately.
Results of this test are labeled for "research purposes only." The result should not be used for treatment or for diagnostic purposes without confirmation of the diagnosis by another medically established diagnostic product or procedure. The performance characteristics were determined by LabCorp.
References
Custom Additional Information
MPO plays an important role in the innate host-defense mechanism of human and animals.
Myeloperoxidate (MPO) is a hemoprotein present in leukocytes and catalyzes the hydrogen peroxide mediated peroxidation of halide ions to produce strong reactive oxidant species such as hypochlorous acid that are of potent antimicrobial activities against a broad range of invading parasites and pathogens. However, MPO-derived reactive oxidants also promote host tissue injury through lipid and protein peroxidations that lead to systemic inflammation.
Specimen
Plasma
Volume
0.5 mL
Minimum Volume
0.2 mL
Container
Lavender-top (EDTA) tube or green-top (heparin) tube
Storage Instructions
Refrigerate.
Causes for Rejection
Specimen type other than heparinized plasma or EDTA plasma; plasma samples left on top of gels for more than eight hours before transferring to separate tubes; specimen received at room temperature
Collection Instructions
Collect whole blood using venipuncture techniques. Gently mix the blood with the anticoagulant by inverting sample tube several times (do not shake).
Place freshly collected blood samples on ice or in a refrigerator 2°C to 8°C immediately, and store them at 2°C to 8°C until separation. Plasma should be physically separated from cells within two hours of collection by centrifugation at 2°C to 8°C. If a refrigerated centrifuge is not available, chill the centrifuge carrier in the refrigerator before use until cold.
Special precaution needs to be taken to ensure transfer of the plasma layer to a polypropylene (not glass) tube while avoiding carryover of any red blood cells or buffy coat white cells. The plasma samples thus prepared may be refrigerated at 2°C to 8°C for five days.
If plasma samples are prepared by gel based vacuum tubes, the plasma samples need to be transferred to separated tubes from the top of the gels immediately after the centrifugation.
Increased levels of MPO may be observed if the plasma samples are left on the top of the gels for more than eight hours before transferring to separate tubes.
Stability Requirements
Temperature | Period |
|---|---|
Refrigerated | 5 days (stability provided by manufacturer or literature reference) |
Frozen | 6 months (stability provided by manufacturer or literature reference) |
Reference Range
• Low CVD Risk: <470 pmol/L
• Moderate Risk: 470–539 pmol/L
• High Risk: >539 pmol/L
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 123006 | Myeloperoxidase (MPO) | 66853-3 | 123007 | Myeloperoxidase (MPO) | pmol/L | 66853-3 |
| Order Code | 123006 | |||||
| Order Code Name | Myeloperoxidase (MPO) | |||||
| Order Loinc | 66853-3 | |||||
| Result Code | 123007 | |||||
| Result Code Name | Myeloperoxidase (MPO) | |||||
| UofM | pmol/L | |||||
| Result LOINC | 66853-3 |