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Hemoglobinopathy Fractionation Cascade

Test Number 121690
Test number copied
CPT 83020

Test Details

Methodology

Capillary electrophoresis (CE); high-pressure liquid chromatography (HPLC); Hgb Solubility: Sodium hydrosulfite reduction

Result Turnaround Time

2 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

Capillary electrophoresis (CE) is performed initially. If an abnormal hemoglobin is detected, appropriate testing will be performed at an additional charge. Depending on findings, one or more reflexive tests may be required in order to provide a clinical interpretation. These tests may include high-pressure liquid chromatography (HPLC) and/or hemoglobin solubility testing.

Use

Diagnose thalassemias and hemoglobin variants

Special Instructions

State the patient's age, ethnic background, MCV, transfusion history, and any other known hematological data on the test request form.

Specimen Requirements

Volume

1.5 mL

Minimum Volume

0.8 mL (Note: This volume does not allow for repeat testing.)

Container

Lavender-top (EDTA) tube

Collection Instructions

To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Reference Range

• Hb A:

– <7 months: Not established

– 7 months to 1 year: 94.6% to 98.5%

− ≥2 years : 96.4% to 98.8%

• Hb F:

− <7 months: Not established

− 7 months to 1 year: 0.1% to 6.8%

− ≥2 years : 0.0% to 2.0%

• Hb S, D, G: 0%

• Hb C, E, O: 0%

• Hb A2:

– <7 months: Not established

– 7 months to 1 year: 1.9% to 2.8%

– ≥2 years: 1.8% to 3.2%

• Hemoglobin solubility: Negative

Storage Instructions

Refrigerate at 2°C to 8°C.

Patient Preparation

Fasting or fluid restriction is not necessary.

Causes for Rejection

Clotted specimen; aged, improperly stored blood samples

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
121690 Hgb Fractionation Cascade 43113-0 121692 Hgb F % 32682-7
121690 Hgb Fractionation Cascade 43113-0 121693 Hgb A % 20572-4
121690 Hgb Fractionation Cascade 43113-0 121694 Hgb A2 % 4552-6
121690 Hgb Fractionation Cascade 43113-0 121695 Hgb S % 32683-5
121690 Hgb Fractionation Cascade 43113-0 121696 Interpretation: 12710-0
Order Code121690
Order Code NameHgb Fractionation Cascade
Order Loinc43113-0
Result Code121692
Result Code NameHgb F
UofM%
Result LOINC32682-7
Order Code121690
Order Code NameHgb Fractionation Cascade
Order Loinc43113-0
Result Code121693
Result Code NameHgb A
UofM%
Result LOINC20572-4
Order Code121690
Order Code NameHgb Fractionation Cascade
Order Loinc43113-0
Result Code121694
Result Code NameHgb A2
UofM%
Result LOINC4552-6
Order Code121690
Order Code NameHgb Fractionation Cascade
Order Loinc43113-0
Result Code121695
Result Code NameHgb S
UofM%
Result LOINC32683-5
Order Code121690
Order Code NameHgb Fractionation Cascade
Order Loinc43113-0
Result Code121696
Result Code NameInterpretation:
UofM
Result LOINC12710-0
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121697 Hgb Solubility 121698 Hgb Solubility 6864-3
Reflex 1
Order Code121697
Order NameHgb Solubility
Result Code121698
Result NameHgb Solubility
UofM
Result LOINC6864-3
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121697 Hgb Solubility 121719 Final Interpretation: 12710-0
Reflex 1
Order Code121697
Order NameHgb Solubility
Result Code121719
Result NameFinal Interpretation:
UofM
Result LOINC12710-0
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121710 Hgb Fractionation by HPLC 121712 Hgb F % 42246-9
Reflex 1
Order Code121710
Order NameHgb Fractionation by HPLC
Result Code121712
Result NameHgb F
UofM%
Result LOINC42246-9
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121710 Hgb Fractionation by HPLC 121713 Hgb A % 42244-4
Reflex 1
Order Code121710
Order NameHgb Fractionation by HPLC
Result Code121713
Result NameHgb A
UofM%
Result LOINC42244-4
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121710 Hgb Fractionation by HPLC 121714 Hgb A2 % 42245-1
Reflex 1
Order Code121710
Order NameHgb Fractionation by HPLC
Result Code121714
Result NameHgb A2
UofM%
Result LOINC42245-1
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121710 Hgb Fractionation by HPLC 121715 Hgb S % 44923-1
Reflex 1
Order Code121710
Order NameHgb Fractionation by HPLC
Result Code121715
Result NameHgb S
UofM%
Result LOINC44923-1
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121710 Hgb Fractionation by HPLC 121716 Hgb C % 44920-7
Reflex 1
Order Code121710
Order NameHgb Fractionation by HPLC
Result Code121716
Result NameHgb C
UofM%
Result LOINC44920-7
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121710 Hgb Fractionation by HPLC 121717 Hgb E % 44922-3
Reflex 1
Order Code121710
Order NameHgb Fractionation by HPLC
Result Code121717
Result NameHgb E
UofM%
Result LOINC44922-3
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121710 Hgb Fractionation by HPLC 121718 Hgb Variant % 42248-5
Reflex 1
Order Code121710
Order NameHgb Fractionation by HPLC
Result Code121718
Result NameHgb Variant
UofM%
Result LOINC42248-5
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121710 Hgb Fractionation by HPLC 121719 Final Interpretation: 12710-0
Reflex 1
Order Code121710
Order NameHgb Fractionation by HPLC
Result Code121719
Result NameFinal Interpretation:
UofM
Result LOINC12710-0
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121720 Hgb Frac by HPLC+Solubility 121698 Hgb Solubility 6864-3
Reflex 1
Order Code121720
Order NameHgb Frac by HPLC+Solubility
Result Code121698
Result NameHgb Solubility
UofM
Result LOINC6864-3
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121720 Hgb Frac by HPLC+Solubility 121712 Hgb F % 42246-9
Reflex 1
Order Code121720
Order NameHgb Frac by HPLC+Solubility
Result Code121712
Result NameHgb F
UofM%
Result LOINC42246-9
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121720 Hgb Frac by HPLC+Solubility 121713 Hgb A % 42244-4
Reflex 1
Order Code121720
Order NameHgb Frac by HPLC+Solubility
Result Code121713
Result NameHgb A
UofM%
Result LOINC42244-4
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121720 Hgb Frac by HPLC+Solubility 121714 Hgb A2 % 42245-1
Reflex 1
Order Code121720
Order NameHgb Frac by HPLC+Solubility
Result Code121714
Result NameHgb A2
UofM%
Result LOINC42245-1
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121720 Hgb Frac by HPLC+Solubility 121715 Hgb S % 44923-1
Reflex 1
Order Code121720
Order NameHgb Frac by HPLC+Solubility
Result Code121715
Result NameHgb S
UofM%
Result LOINC44923-1
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121720 Hgb Frac by HPLC+Solubility 121716 Hgb C % 44920-7
Reflex 1
Order Code121720
Order NameHgb Frac by HPLC+Solubility
Result Code121716
Result NameHgb C
UofM%
Result LOINC44920-7
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121720 Hgb Frac by HPLC+Solubility 121717 Hgb E % 44922-3
Reflex 1
Order Code121720
Order NameHgb Frac by HPLC+Solubility
Result Code121717
Result NameHgb E
UofM%
Result LOINC44922-3
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121720 Hgb Frac by HPLC+Solubility 121718 Hgb Variant % 42248-5
Reflex 1
Order Code121720
Order NameHgb Frac by HPLC+Solubility
Result Code121718
Result NameHgb Variant
UofM%
Result LOINC42248-5
Reflex Table for Interpretation:
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 121720 Hgb Frac by HPLC+Solubility 121719 Final Interpretation: 12710-0
Reflex 1
Order Code121720
Order NameHgb Frac by HPLC+Solubility
Result Code121719
Result NameFinal Interpretation:
UofM
Result LOINC12710-0