Test Details
Methodology
Protein electrophoresis: agarose media; free κ and λ light chains: turbidimetric quantitation
Result Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Serum protein electrophoresis with relative percentages and quantitation of albumin; α1-, α2-, β-, and γ-globulin fractions; graph of fractions; quantitation of total protein and M-spike if present; quantitation of serum free κ and λ light chains plus κ:λ ratio
Use
Detect and identify monoclonal immunoglobulin gammopathies
Custom Additional Information
Elevated serum levels of monoclonal FLC are associated with malignant plasma cell proliferation (eg, multiple myeloma), primary amyloidosis, and light chain deposition disease. Raised serum levels of polyclonal FLC may be associated with autoimmune diseases such as systemic lupus erythematosus.
Specimen Requirements
Specimen
Serum
Volume
2.5 mL
Minimum Volume
1.2 mL
Container
Red-top tube or gel-barrier tube
Collection Instructions
Sample should be allowed to clot and the serum separated as soon as possible to prevent hemolysis.
Storage Instructions
PE: Stable when covered at room temperature 25°C for four days; stable refrigerated at 2°C to 8°C for two weeks; stable frozen at -20°C for six months.
FLC: Refrigerate; samples are stable for 21 days at 2°C to 8°C. For longer storage, freeze at -20°C.
Causes for Rejection
Lipemia; hemolysis; plasma specimen
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 121210 | PE and FLC, Serum | 001073 | Protein, Total | g/dL | 2885-2 | |
| 121210 | PE and FLC, Serum | 001488 | Albumin | g/dL | 2862-1 | |
| 121210 | PE and FLC, Serum | 001489 | Alpha-1-Globulin | g/dL | 2865-4 | |
| 121210 | PE and FLC, Serum | 001490 | Alpha-2-Globulin | g/dL | 2868-8 | |
| 121210 | PE and FLC, Serum | 001491 | Beta Globulin | g/dL | 2871-2 | |
| 121210 | PE and FLC, Serum | 001492 | Gamma Globulin | g/dL | 2874-6 | |
| 121210 | PE and FLC, Serum | 001493 | M-Spike | g/dL | 33358-3 | |
| 121210 | PE and FLC, Serum | 011052 | Globulin, Total | g/dL | 10834-0 | |
| 121210 | PE and FLC, Serum | 011149 | A/G Ratio | 1759-0 | ||
| 121210 | PE and FLC, Serum | 011150 | Please note: | 77202-0 | ||
| 121210 | PE and FLC, Serum | 001498 | 11502-2 | |||
| 121210 | PE and FLC, Serum | 121138 | Free Kappa Lt Chains,S | mg/L | 36916-5 | |
| 121210 | PE and FLC, Serum | 121139 | Free Lambda Lt Chains,S | mg/L | 33944-0 | |
| 121210 | PE and FLC, Serum | 121141 | Kappa/Lambda Ratio,S | 48378-4 | ||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 001073 | |||||
| Result Code Name | Protein, Total | |||||
| UofM | g/dL | |||||
| Result LOINC | 2885-2 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 001488 | |||||
| Result Code Name | Albumin | |||||
| UofM | g/dL | |||||
| Result LOINC | 2862-1 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 001489 | |||||
| Result Code Name | Alpha-1-Globulin | |||||
| UofM | g/dL | |||||
| Result LOINC | 2865-4 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 001490 | |||||
| Result Code Name | Alpha-2-Globulin | |||||
| UofM | g/dL | |||||
| Result LOINC | 2868-8 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 001491 | |||||
| Result Code Name | Beta Globulin | |||||
| UofM | g/dL | |||||
| Result LOINC | 2871-2 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 001492 | |||||
| Result Code Name | Gamma Globulin | |||||
| UofM | g/dL | |||||
| Result LOINC | 2874-6 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 001493 | |||||
| Result Code Name | M-Spike | |||||
| UofM | g/dL | |||||
| Result LOINC | 33358-3 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 011052 | |||||
| Result Code Name | Globulin, Total | |||||
| UofM | g/dL | |||||
| Result LOINC | 10834-0 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 011149 | |||||
| Result Code Name | A/G Ratio | |||||
| UofM | ||||||
| Result LOINC | 1759-0 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 011150 | |||||
| Result Code Name | Please note: | |||||
| UofM | ||||||
| Result LOINC | 77202-0 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 001498 | |||||
| Result Code Name | ||||||
| UofM | ||||||
| Result LOINC | 11502-2 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 121138 | |||||
| Result Code Name | Free Kappa Lt Chains,S | |||||
| UofM | mg/L | |||||
| Result LOINC | 36916-5 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 121139 | |||||
| Result Code Name | Free Lambda Lt Chains,S | |||||
| UofM | mg/L | |||||
| Result LOINC | 33944-0 | |||||
| Order Code | 121210 | |||||
| Order Code Name | PE and FLC, Serum | |||||
| Order Loinc | ||||||
| Result Code | 121141 | |||||
| Result Code Name | Kappa/Lambda Ratio,S | |||||
| UofM | ||||||
| Result LOINC | 48378-4 |