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HIV p24 Antigen/Antibody With Reflex to Confirmation

Test Number 083935
Test number copied
CPT

87389. If reflex testing is performed, concomitant CPT codes/charges will apply.
Synonyms
  • HIV Antigen/Antibody
  • HIV-1
  • HIV-2
  • Human Immunodeficiency Virus
  • p24
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  • Updated on 02/28/2025

Test Details

Methodology

(1) HIV antigen/antibody enzyme immunoassay (EIA) screen for antibodies to HIV-1 and HIV-2 and HIV-1 p24 antigen: Reflex to supplementary differentiation assay; (2) Screen positive with negative antibody typing: Reflex to qualitative nucleic acid amplification (NAA)

Result Turnaround Time

1 - 2 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

For more information, please view the literature below.

Human Immunodeficiency Virus 1/O/2 Cascade: Fourth-generation Antigen/Antibody Detection

HIV Screening and Diagnosis White Paper

Test Includes

HIV p24 antigen/antibody screen for HIV-1 antibody, HIV-2 antibody, and HIV-1 p24 antigen by EIA; reflex of EIA positive specimens to a HIV-1/HIV-2 antibody differentiation assay; specimens positive by the screening assay but negative by the antibody differentiation assay reflex to HIV-1/HIV-2 RNA testing by qualitative nucleic acid amplification (NAA).

Use

CDC-recommended algorithm for laboratory diagnosis of HIV infection, including acute and primary infection

Special Instructions

This test requires a dedicated, unopened, gel-barrier tube sent directly to the testing facility.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

This test requires a dedicated, unopened, gel-barrier tube or red-top tube sent directly to the testing facility.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

This test requires a dedicated, unopened, gel-barrier tube sent directly to the testing facility.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Limitations

Reactive (repeatedly) specimens must be investigated by additional supplemental tests. A negative test for an individual does not preclude exposure to or infection with HIV-1 and/or HIV-2. Negative results can occur if the quantity of the marker present in the sample is too low for detection by the assay or if the marker is not present during the stage of disease in which a sample is collected.

References

Association of Public Health Laboratories. Suggested Reporting Language for the HIV Laboratory Diagnostic Testing Algorithm. APHL 2019. APHL Web site. https://www.aphl.org/aboutAPHL/publications/Documents/ID-2019Jan-HIV-Lab-Test-Suggested-Reporting-Language.pdf. Accessed November 2019.
Centers for Disease Control and Prevention. Laboratory testing for the diagnosis of HIV infection : updated recommendations. CDC Stacks Web site. https://stacks.cdc.gov/view/cdc/23447. Accessed November 2019.
Clinical and Laboratory Standards Institute. Criteria for Laboratory Testing and Diagnosis of the Human Immunodeficiency Virus Infection, Approved Guideline. M53-A. Wayne, Pa: CLSI; 2011.

Specimen Requirements

Specimen

Serum

Volume

2 mL

Minimum Volume

1 mL (Note: This volume does not allow for repeat testing.)

Container

Gel-barrier tube

Gel-barrier tube or red-top tube

Gel-barrier tube

Collection Instructions

Usual blood collection technique (do not freeze serum gel tubes; send to the lab refrigerated)

Stability Requirements

TemperaturePeriod
Refrigerated7 days (stability provided by manufacturer or literature reference)
Frozen14 days (stability provided by manufacturer or literature reference) *This stability information is intended for laboratory storage. Do not freeze serum gel tubes; send to the lab refrigerated.
Freeze/thaw cyclesStable x5 (stability provided by manufacturer or literature reference)

Storage Instructions

Refrigerate.

Causes for Rejection

Specimen aliquot; specimen grossly hemolyzed; specimen in container other than those listed; received outside of specimen and/or storage and/or labeling requirements

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
083935 HIV Ab/p24 Ag with Reflex 75622-1 083936 HIV Ab/p24 Ag Screen 56888-1
Order Code083935
Order Code NameHIV Ab/p24 Ag with Reflex
Order Loinc75622-1
Result Code083936
Result Code NameHIV Ab/p24 Ag Screen
UofM
Result LOINC56888-1
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083941 HIV 1 Ab 68961-2
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083941
Result NameHIV 1 Ab
UofM
Result LOINC68961-2
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083942 HIV 2 Ab 81641-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083942
Result NameHIV 2 Ab
UofM
Result LOINC81641-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3
Reflex Table for HIV Ab/p24 Ag Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 083940 HIV 1/2 Ab Differentiation 083954 Interpretation: 80203-3
Reflex 1
Order Code083940
Order NameHIV 1/2 Ab Differentiation
Result Code083954
Result NameInterpretation:
UofM
Result LOINC80203-3