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Rotavirus, Direct Detection Immunoassay

CPT 87425

Test Details

Methodology

Immunoassay (IA)

Result Turnaround Time

1 - 4 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

For more information, please view the literature below.

Microbiology Specimen Collection and Transport Guide

Use

Detect rotavirus in stools of patients suspected of having viral gastroenteritis

Limitations

Quality of specimens cannot be evaluated, and specimens are collected randomly.

Footnotes

1. Christensen ML, Howard C. Viruses causing gastroenteritis. In: Balows A, Hausler WJ, et al, eds. Manual of Clinical Microbiology. 5th ed. Washington, DC: ASM Press;1991:950-958.

References

Christensen ML. Human viral gastroenteritis. Clin Microbiol Rev. 1989 Jan; 2(1):51-89. 2644024
Gray LD. Novel viruses associated with gastroenteritis. Clin Microbiol Newslet. 1991; 13(18):137-144.

Custom Additional Information

Rotavirus is an extremely common cause of pediatric gastroenteritis. The illness is most common in winter, is highly contagious, involves five to eight days of diarrhea, and is rarely fatal.1 Patients should also be evaluated for possible bacterial gastroenteritis. Outbreaks are seen among children in daycare centers.

Specimen Requirements

Specimen

Stool

Volume

2 mL liquid stool or 1 g semiformed stool

Container

Empty stool transport vial or screw-cap container, sterile no additives

Collection Instructions

Collect as soon as possible after onset of disease, preferably three to five days after onset. Collection from diapered children can be facilitated by using a disposable diaper “inside out” or by using a “Saran wrap” diaper to capture the next stool specimen.

Reference Range

Negative

Storage Instructions

Refrigerate at 2°C to 8°C immediately after collection.

Causes for Rejection

Specimen received in grossly leaking transport container; dry specimen; specimen submitted in fixative or additive; bacterial swab specimen; diapers; unlabeled specimen or name discrepancy between specimen and request label; specimen transported at room temperature; other inappropriate specimen transport devices; specimen received after prolonged delay (usually more than 72 hours); specimen in transport media or preservative

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
006866 Rotavirus Ag, EIA 5880-0 006866 Rotavirus Ag, EIA 5880-0
Order Code006866
Order Code NameRotavirus Ag, EIA
Order Loinc5880-0
Result Code006866
Result Code NameRotavirus Ag, EIA
UofM
Result LOINC5880-0