Test Details
Methodology
Immunochemiluminometric assay (ICMA)
Result Turnaround Time
1 - 2 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This assay is used to detect total antibodies (IgG and IgM) against Hepatitis A virus (HAV) and may be helpful when assessing HAV immunity. The detection of total antibodies cannot differentiate between vaccination, previously resolved infection, or active infection. If active hepatitis A infection is suspected, HAV IgM testing should be performed. Hepatitis A is a vaccine-preventable disease of the liver caused by the Hepatitis A virus (HAV). HAV is transmitted via the fecal-oral route, usually from direct person-to-person contact or consumption of contaminated food or water. Hepatitis A is an acute, self-limited disease that does not result in chronic infection. HAV IgG antibodies produced in response to vaccination and/or HAV infection persist for life and protect against reinfection.
Special Instructions
This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.
Limitations
This assay has not been FDA cleared or approved for the screening of blood or plasma donors. Assay performance characteristics have not been established for immunocompromised or immunosuppressed individuals. This assay does not differentiate IgG and IgM.
Custom Additional Information
See table.
HAV antibody testing interpretation chartHAV Total Antibody | HAV IgM | Comments |
|---|---|---|
Negative | Not done | No evidence of vaccination or previous infection; Susceptible to Hepatitis A infection |
Positive | Not done | Consistent with recent or remote Hepatitis A infection or antibody response to HAV vaccination |
Positive | Negative | Consistent with resolved Hepatitis A infection or antibody response to HAV vaccination |
Positive | Positive | Consistent with active Hepatitis A infection |
Specimen Requirements
Specimen
Serum or plasma
Volume
1 mL
Minimum Volume
0.4 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube, gel-barrier tube or lavender-top (EDTA) tube
Collection Instructions
If tube other than a gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube. Do not freeze gel-barrier tube (pour off serum first).
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Reference Range
Negative
Storage Instructions
Room temperature
Causes for Rejection
Non-EDTA plasma specimen; PST gel-barrier tube; grossly hemolyzed specimens
References
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 006726 | Hep A Ab, Total | 13951-9 | 006726 | Hep A Ab, Total | 13951-9 | |
| Order Code | 006726 | |||||
| Order Code Name | Hep A Ab, Total | |||||
| Order Loinc | 13951-9 | |||||
| Result Code | 006726 | |||||
| Result Code Name | Hep A Ab, Total | |||||
| UofM | ||||||
| Result LOINC | 13951-9 |