Test Details
Methodology
Immunochemiluminometric assay (ICMA)
Result Turnaround Time
1 - 3 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
The HBe antigen assay, in conjunction with other serological and clinical information, can be used for the determination and management of chronic hepatitis B infection. This assay should not be used for the diagnosis of acute Hepatitis B infection.
Special Instructions
This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.
Limitations
This assay has not been FDA-approved for the diagnosis of individuals with acute hepatitis B infection.
Assay performance characteristics have not been established for testing of children less than 17 years of age or pregnant women or in populations of immunocompromised or immunosuppressed patients.
This assay has not been licensed for the screening of blood, plasma, and tissue donors.
Custom Additional Information
HBe antigen becomes detectable shortly after HBsAg is detectable and is an indicator of active infection and replicating virus. The disappearance of HBeAg and the appearance of anti-HBe together with other HBV markers allow the clinician to determine a prognosis, and to follow the progression of the disease from acute to chronic or recovered status.
Specimen Requirements
Specimen
Serum or plasma
Volume
1.5 mL
Minimum Volume
0.5 mL (Note: This volume does not allow for repeat testing.)
Container
Gel-barrier tube or lavender-top (EDTA) tube
Collection Instructions
If tube other than a gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube.
Stability Requirements
Temperature | Period |
|---|---|
Room Temperature | 7 days (stability provided by manufacturer or literature reference) |
Refrigerated | 7 days (stability provided by manufacturer or literature reference) |
Frozen | 1 year (stability provided by manufacturer or literature reference) |
Freeze/thaw cycles | Stable x3 (stability provided by manufacturer or literature reference) |
Reference Range
Negative
Storage Instructions
Room temperature
Causes for Rejection
Non-EDTA plasma specimen; heat-inactivated specimens; cord blood; cadaver specimens; or body fluids other than serum or EDTA plasma
References
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 006619 | Hep Be Ag | 13954-3 | 006619 | Hep Be Ag | 13954-3 | |
| Order Code | 006619 | |||||
| Order Code Name | Hep Be Ag | |||||
| Order Loinc | 13954-3 | |||||
| Result Code | 006619 | |||||
| Result Code Name | Hep Be Ag | |||||
| UofM | ||||||
| Result LOINC | 13954-3 |