Test Details
Methodology
Immunochemiluminometric assay (ICMA)
Result Turnaround Time
1 - 2 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test is useful for quantitative (i.e., titer) evaluation of possible immunity in individuals who are at increased risk for exposure to hepatitis B (i.e., hemodialysis unit personnel, venipuncturists, etc.). This test can be used to evaluate the need for hepatitis B immune globulin after needlestick injury, to evaluate the need for hepatitis B vaccine and to follow immune status after hepatitis B vaccination.
Limitations
Presence of anti-HBs is not an absolute indicator of resolved hepatitis infection nor of protection from future infection. Since there are different serologic subtypes of hepatitis B virus, it is possible (and has been reported) for a patient to have antibody to one surface antigen type and to be acutely infected with a virus of a different subtype. Thus, a patient may have coexisting HBsAg and anti-HBs. Transfused individuals or hemophiliacs receiving plasma components may give false-positive tests for antibody to hepatitis B surface antigen.
Specimen Requirements
Specimen
Serum or plasma
Volume
1 mL
Minimum Volume
0.5 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube, gel-barrier tube, or lavender-top (EDTA) tube
Collection Instructions
If tube other than a gel-barrier tube is used, transfer separated serum or plasma to a plastic transport tube.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Reference Range
• Immunity: >10.0 mIU/mL
Storage Instructions
Room temperature
Causes for Rejection
Non-EDTA plasma specimen; PST gel-barrier tube
References
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 006530 | Hepatitis B Surf Ab Quant | 16935-9 | 006531 | Hepatitis B Surf Ab Quant | mIU/mL | 16935-9 |
| Order Code | 006530 | |||||
| Order Code Name | Hepatitis B Surf Ab Quant | |||||
| Order Loinc | 16935-9 | |||||
| Result Code | 006531 | |||||
| Result Code Name | Hepatitis B Surf Ab Quant | |||||
| UofM | mIU/mL | |||||
| Result LOINC | 16935-9 |