Test Details
Methodology
Hemagglutination
Result Turnaround Time
2 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
Determine the Lewis phenotype
Limitations
Presence of potent cold agglutinins, positive direct antiglobulin test, and (in some cases) bacteremia may interfere with test interpretation.
Custom Additional Information
The occurrence of the Lea antigen is 22% in Caucasians and 23% in Blacks. Anti-Lea does not cause hemolytic disease of the fetus and newborn, as the Lewis antigens are not expressed on cord RBC.
The occurrence of the Leb antigen is 72% in Caucasians and 55% in Blacks. Anti-Leb does not cause hemolytic disease of the fetus and newborn, as the Lewis antigens are not expressed on cord RBC.
Specimen Requirements
Specimen
Whole blood
Volume
1 mL
Minimum Volume
0.5 mL (Note: This volume does not allow for repeat testing.)
Container
Lavender-top (EDTA) tube
Storage Instructions
Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze red cells.
Causes for Rejection
Gross hemolysis; bacterial or other contamination
References
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 006034 | Lea/Leb RBC Antigen Typing | 93914-0 | 006032 | Lea | 1115-5 | |
| 006034 | Lea/Leb RBC Antigen Typing | 93914-0 | 006033 | Leb | 1121-3 | |
| Order Code | 006034 | |||||
| Order Code Name | Lea/Leb RBC Antigen Typing | |||||
| Order Loinc | 93914-0 | |||||
| Result Code | 006032 | |||||
| Result Code Name | Lea | |||||
| UofM | ||||||
| Result LOINC | 1115-5 | |||||
| Order Code | 006034 | |||||
| Order Code Name | Lea/Leb RBC Antigen Typing | |||||
| Order Loinc | 93914-0 | |||||
| Result Code | 006033 | |||||
| Result Code Name | Leb | |||||
| UofM | ||||||
| Result LOINC | 1121-3 |