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Synonyms
- Indirect Antiglobulin Test
- Indirect Coombs'
Test Includes
Red cell antibody screening. Note: Identification of antibody(ies) and titer of clinically significant antibody(ies) will be automatically performed at an additional charge if the antibody screen is positive.
Expected Turnaround Time
1 - 2 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Whole blood (Note: This should not be a shared specimen. If antibody identification is necessary, the entire volume of this lavender-top tube will be required.)
Volume
4 mL
Minimum Volume
1 mL (Note: This minimum amount will not allow for antibody identification if the antibody screen is positive.)
Container
Lavender-top (EDTA) tube
Storage Instructions
Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze red cells.
Causes for Rejection
Gross hemolysis; bacterial or other contamination
Test Details
Use
Detect atypical IgG antibodies prior to transfusion or during pregnancy. The technique is designed specifically to detect IgG antibodies, though on occasion, some IgM antibodies may also be detected. Antibodies detected by the antibody screen will be subsequently identified, and a titer performed if the antibody identified is considered to be clinically significant during pregnancy.
Limitations
No antibody screening test will detect all antibodies. This method may not detect IgM antibodies or antibodies to low frequency antigens. Extremely low-titered antibodies may be detected but may be too weak to identify.
Methodology
Antiglobulin test
Contraindications
Specimens known to test positive for red cell antibody screening should not be submitted for antibody screen testing. In order to facilitate proper routing and testing, Antibody Identification (006213) should be ordered. This test should not be ordered for the husband or partner of a prenatal patient since antibodies detected in the serum or plasma of these individuals are irrelevant during pregnancy.
Reference Interval
Negative
References
Fung MK, Grossman JB, Hillyer CD, Westoff CM, eds. AABB Technical Manual. 18th ed. Bethesda, Md: American Association of Blood Banks;2014.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 006015 | Antibody Screen | 890-4 | 006015 | Antibody Screen | 890-4 |
| Reflex Table for Antibody Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 114041 | Ab Scr+Antibody ID | 114033 | Antibody Screen | 890-4 | |
| Reflex Table for Antibody Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 114041 | Ab Scr+Antibody ID | 019786 | Antibody Id. #1 | 888-8 | |
| Reflex 2 | 114108 | Ab Scr+Antibody ID | 114034 | Antibody Screen | 890-4 | |
| Reflex Table for Antibody Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 114041 | Ab Scr+Antibody ID | 019786 | Antibody Id. #1 | 888-8 | |
| Reflex 2 | 114108 | Ab Scr+Antibody ID | 114035 | Antibody Id. #1 | 888-8 | |
| Reflex Table for Antibody Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 114041 | Ab Scr+Antibody ID | 019786 | Antibody Id. #1 | 888-8 | |
| Reflex 2 | 114108 | Ab Scr+Antibody ID | 114036 | Coombs Titer #1 | 50970-3 | |
| Reflex Table for Antibody Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 114041 | Ab Scr+Antibody ID | 019786 | Antibody Id. #1 | 888-8 | |
| Reflex 2 | 114108 | Ab Scr+Antibody ID | 114037 | Antibody Id. #2 | 888-8 | |
| Reflex Table for Antibody Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 114041 | Ab Scr+Antibody ID | 019786 | Antibody Id. #1 | 888-8 | |
| Reflex 2 | 114108 | Ab Scr+Antibody ID | 114038 | Coombs Titer #2 | 50970-3 | |
| Reflex Table for Antibody Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 114041 | Ab Scr+Antibody ID | 019802 | Coombs Titer #1 | 50970-3 | |
| Reflex Table for Antibody Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 114041 | Ab Scr+Antibody ID | 019810 | Antibody Id. #2 | 888-8 | |
| Reflex Table for Antibody Screen | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 114041 | Ab Scr+Antibody ID | 019836 | Coombs Titer #2 | 50970-3 | |
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