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Hemoglobin (Hb) Solubility With Reflex to Hemoglobinopathy Fractionation Cascade, Blood
CPT:
85660. If reflex testing is performed, concomitant CPT codes/charges will apply.
Synonyms
- Hb Solubility
- Sickle Cell Preparation
- Sickle Cell Solubility Test
- Sickle Cell Test
- Sickle Prep
- Sickledex™
Test Includes
Hemoglobin (Hb) solubility and automatic reflex to hemoglobin (Hb) fractionation blood (at additional charge), if positive.
Expected Turnaround Time
2 - 3 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
Specimen Requirements
Specimen
Whole blood
Volume
1 mL
Minimum Volume
0.6 mL (Note: This volume does not allow for repeat testing.)
Container
Lavender-top (EDTA) tube; capillary puncture: three microhematocrit tubes
Note: Because this profile reflexes to Hemoglobinopathy Fractionation Cascade [121690] when positive, only a Lavender-top (EDTA) tube is allowed.
Collection
To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.
Storage Instructions
Room temperature
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Patient Preparation
No preliminary fluid or food restriction is required.
Causes for Rejection
Specimen other than whole blood received; clotted specimen
Test Details
Use
Qualitative determination of presence of hemoglobin S; detect sickling hemoglobins; evaluate hemolytic anemia, undiagnosed hereditary anemia with morphologic (sickle-like) abnormalities on peripheral blood smear
Limitations
Methodology
Sodium hydrosulfite reduction
Reference Interval
Negative
Additional Information
References
Chehab FF, Kan YW. Detection of sickle cell anaemia mutation by colour DNA amplification. Lancet. 1990 Jan 6; 335(8680):15-17. 1967329
Miale JB. Laboratory Medicine: Hematology. 6th ed. St Louis, Mo: Mosby-Year Book Inc;1982:624-625.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 005330 | HB Solu + Rflx Frac | 6864-3 | 005224 | Hemoglobin (Hgb) Solubility | 6864-3 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121692 | Hgb F | % | 32682-7 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121693 | Hgb A | % | 20572-4 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121694 | Hgb A2 | % | 4552-6 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121695 | Hgb S | % | 32683-5 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121697 | Hgb Solubility | 121698 | Hgb Solubility | 6864-3 | |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121697 | Hgb Solubility | 121719 | Final Interpretation: | 12710-0 | |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121710 | Hgb Fractionation by HPLC | 121712 | Hgb F | % | 42246-9 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121710 | Hgb Fractionation by HPLC | 121713 | Hgb A | % | 42244-4 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121710 | Hgb Fractionation by HPLC | 121714 | Hgb A2 | % | 42245-1 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121710 | Hgb Fractionation by HPLC | 121715 | Hgb S | % | 44923-1 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121710 | Hgb Fractionation by HPLC | 121716 | Hgb C | % | 44920-7 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121710 | Hgb Fractionation by HPLC | 121717 | Hgb E | % | 44922-3 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121710 | Hgb Fractionation by HPLC | 121718 | Hgb Variant | % | 42248-5 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121710 | Hgb Fractionation by HPLC | 121719 | Final Interpretation: | 12710-0 | |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121720 | Hgb Frac by HPLC+Solubility | 121698 | Hgb Solubility | 6864-3 | |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121720 | Hgb Frac by HPLC+Solubility | 121712 | Hgb F | % | 42246-9 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121720 | Hgb Frac by HPLC+Solubility | 121713 | Hgb A | % | 42244-4 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121720 | Hgb Frac by HPLC+Solubility | 121714 | Hgb A2 | % | 42245-1 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121720 | Hgb Frac by HPLC+Solubility | 121715 | Hgb S | % | 44923-1 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121720 | Hgb Frac by HPLC+Solubility | 121716 | Hgb C | % | 44920-7 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121720 | Hgb Frac by HPLC+Solubility | 121717 | Hgb E | % | 44922-3 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121720 | Hgb Frac by HPLC+Solubility | 121718 | Hgb Variant | % | 42248-5 |
| Reflex Table for Hemoglobin (Hgb) Solubility | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 121690 | Hgb Fractionation Cascade | 121696 | Interpretation: | 12710-0 | |
| Reflex 2 | 121720 | Hgb Frac by HPLC+Solubility | 121719 | Final Interpretation: | 12710-0 | |
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