Test Details
Methodology
Kinetic − 340 nm
Result Turnaround Time
3 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
5′ nucleotidase is used to investigate the origin of increased serum alkaline phosphatase. It is a liver-related enzyme used to work up cholestatic/biliary obstruction. It parallels the increases of alkaline phosphatase and leucine aminopeptidase in hepatobiliary diseases, but is not usually elevated in skeletal disorders such as Paget disease of bone. It is increased with metastatic neoplasia in liver and in primary biliary cirrhosis, biliary obstruction from calculi and tumor.
Specimen Requirements
Specimen
Serum (EDTA or heparin plasma may be used.)
Volume
1 mL
Minimum Volume
0.2 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube for serum; lavender-top (EDTA) tube or green-top (heparin) tube for plasma
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Reference Range
See table.
Age | Male (IU/L) | Female (IU/L) |
|---|---|---|
0 to 1 y | 0−6 | 0−18 |
2 to 12 y | 0−7 | 0–7 |
13 to 17 y | 0−5 | 0−5 |
18 to 30 y | 0–5 | 0–7 |
31 to 50 y | 0−10 | 0−10 |
51 to 70 y | 0−11 | 0−11 |
71 to 80 y | 0−13 | 0−13 |
>80 y | 0−18 | 0−15 |
Storage Instructions
Room temperature
Causes for Rejection
Hemolysis
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 001701 | 5' Nucleotidase | 1690-7 | 001701 | 5' Nucleotidase | IU/L | 1690-7 |
| Order Code | 001701 | |||||
| Order Code Name | 5' Nucleotidase | |||||
| Order Loinc | 1690-7 | |||||
| Result Code | 001701 | |||||
| Result Code Name | 5' Nucleotidase | |||||
| UofM | IU/L | |||||
| Result LOINC | 1690-7 |