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Immunofixation (IFE), Serum and Protein Electrophoresis, Serum

Test Number 001495
Test number copied
CPT 82784(x3) (IgG, IgA, IgM); 84155; 84165 (protein electrophoretic fractionation and quantitation); 86334 (immunofixation electrophoresis)
Synonyms
  • Globulins, Serum
  • IEP, Serum
  • IFE, Serum and Protein Electrophoresis, Serum
  • Immunoelectrophoresis, Serum
  • Protein IEP

Test Details

Methodology

Electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chains (IFE); protein electrophoresis on agarose media; turbidimetric quantitation of IgA, IgG, and IgM

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

For more information, please view the literature below.

Published Plasma Cell Dyscrasias Selection Table

Test Includes

Quantitation of immunoglobulins IgA, IgG, and IgM; IFE interpretation of heavy- and light-chain patterns; serum protein electrophoresis

Use

Detect and identify monoclonal immunoglobulin gammopathies

Special Instructions

State the patient's age and clinical diagnosis on the test request form.

Custom Additional Information

Diagnose myeloma, macroglobulinemia of Waldenström. Immunofixation is used to evaluate monoclonal gammopathy (M protein) found on serum protein electrophoresis; evaluate amyloidosis. Has application to the evaluation of lymphoproliferative disease (malignant lymphoma and others) and collagen disease in general. Of use in the diagnosis and characterization of immune deficient and dysgammaglobulinemic states.

Specimen Requirements

Specimen

Serum

Volume

5 mL

Minimum Volume

2 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Collection Instructions

Separate serum from cells.

Stability Requirements

Temperature

Period

Room temperature

3 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Reference Range

Normal pattern

Storage Instructions

Refrigerate.

Patient Preparation

Overnight fasting is preferred, but not required.

Causes for Rejection

Plasma sample

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
001495 IFE and PE, Serum 001776 Immunoglobulin G, Qn, Serum mg/dL 2465-3
001495 IFE and PE, Serum 001784 Immunoglobulin A, Qn, Serum mg/dL 2458-8
001495 IFE and PE, Serum 001792 Immunoglobulin M, Qn, Serum mg/dL 2472-9
001495 IFE and PE, Serum 001073 Protein, Total g/dL 2885-2
001495 IFE and PE, Serum 149520 Albumin g/dL 2862-1
001495 IFE and PE, Serum 149521 Alpha-1-Globulin g/dL 2865-4
001495 IFE and PE, Serum 149522 Alpha-2-Globulin g/dL 2868-8
001495 IFE and PE, Serum 149523 Beta Globulin g/dL 2871-2
001495 IFE and PE, Serum 149524 Gamma Globulin g/dL 2874-6
001495 IFE and PE, Serum 149525 M-Spike g/dL 33358-3
001495 IFE and PE, Serum 149530 Globulin, Total g/dL 2336-6
001495 IFE and PE, Serum 149531 A/G Ratio 1759-0
001495 IFE and PE, Serum 149519 Immunofixation Result, Serum 13169-8
001495 IFE and PE, Serum 149534 Please note: 77202-0
001495 IFE and PE, Serum 001498 PDF 11502-2
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code001776
Result Code NameImmunoglobulin G, Qn, Serum
UofMmg/dL
Result LOINC2465-3
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code001784
Result Code NameImmunoglobulin A, Qn, Serum
UofMmg/dL
Result LOINC2458-8
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code001792
Result Code NameImmunoglobulin M, Qn, Serum
UofMmg/dL
Result LOINC2472-9
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code001073
Result Code NameProtein, Total
UofMg/dL
Result LOINC2885-2
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code149520
Result Code NameAlbumin
UofMg/dL
Result LOINC2862-1
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code149521
Result Code NameAlpha-1-Globulin
UofMg/dL
Result LOINC2865-4
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code149522
Result Code NameAlpha-2-Globulin
UofMg/dL
Result LOINC2868-8
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code149523
Result Code NameBeta Globulin
UofMg/dL
Result LOINC2871-2
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code149524
Result Code NameGamma Globulin
UofMg/dL
Result LOINC2874-6
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code149525
Result Code NameM-Spike
UofMg/dL
Result LOINC33358-3
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code149530
Result Code NameGlobulin, Total
UofMg/dL
Result LOINC2336-6
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code149531
Result Code NameA/G Ratio
UofM
Result LOINC1759-0
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code149519
Result Code NameImmunofixation Result, Serum
UofM
Result LOINC13169-8
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code149534
Result Code NamePlease note:
UofM
Result LOINC77202-0
Order Code001495
Order Code NameIFE and PE, Serum
Order Loinc
Result Code001498
Result Code NamePDF
UofM
Result LOINC11502-2
Reflex Table for Immunofixation Result, Serum
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 149532 Immunofixation Interp.,Sr 149532 Immunofixation Interp.,Sr 13169-8
Reflex 1
Order Code149532
Order NameImmunofixation Interp.,Sr
Result Code149532
Result NameImmunofixation Interp.,Sr
UofM
Result LOINC13169-8