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Gastrointestinal Profile, Stool, PCR

Test Number 183480
Test number copied
CPT

87507
Synonyms
  • Stool NAAT
  • Stool PCR
  • Stool Pathogen

Test Details

Methodology

Polymerase chain reaction (PCR)

Result Turnaround Time

2 - 3 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

Test Includes

Adenovirus F 40/41; astrovirus; Campylobacter; Clostridium difficile toxin A/B; Cryptosporidium; Cyclospora cayetanensis; E coli O157; Entamoeba histolytica; enteroaggregative E coli (EAEC); enteropathogenic E coli (EPAC); enterotoxigenic E coli (ETEC) lt/st; Giardia lamblia; norovirus GI/GII; Plesiomonas shigelloides; rotavirus A; Salmonella; Sapovirus; Shiga-like toxin-producing E coli (STEC) stx1/stx2; Shigella/enteroinvasive E coli (EIEC); Vibrio; Vibrio cholerae; Yersinia enterocolitica

Use

Gastrointestinal disease can be caused by many agents, including bacteria, viruses and parasites. Culture for bacteria will not pick up the viruses or parasites. Tests for parasites will not detect the viruses or the bacteria. This GI profile is a multiplexed nucleic acid test intended for the simultaneous qualitative detection and identification of nucleic acids from multiple bacteria, viruses and parasites directly from stool samples in Cary-Blair transport media obtained from individuals with signs and/or symptoms of gastrointestinal infection.

Special Instructions

Introduce stool into orange Para-Pak® vial in small amounts such that level does not exceed fill-line on label. Cap securely and shake vial to distribute sample into the Cary-Blair preservative.

Limitations

The performance of this test has not been established for patients without signs and symptoms of gastrointestinal illness. Virus, bacteria and parasite nucleic acid may persist in vivo independently of organism viability. Additionally, some organisms may be carried asymptomatically. Detection of organism targets does not imply that the corresponding organisms are infectious or are the causative agents for clinical symptoms. The detection of organism nucleic acid is dependent upon proper sample collection, handling, transportation, storage and preparation. Failure to observe proper procedures in any one of these steps can lead to incorrect results. There is a risk of false-positive and false-negative results caused by improperly collected, transported or and handled specimens.

References

Buss SN, Leber A, Fey PD, et al. Multicenter evaluation of the BioFire FilmArray® gastrointestinal panel for the etiologic diagnosis of infectious gastroenteritis. J Clin Microbiol. 2015 Mar;53(3):915-925.25588652
FilmArray Gastrointestinal Panel (GI) Instruction Booklet (RFIT-PRT-0143-01), BioFire Diagnostics, LLC.
Rand KH, Tremblay EE, Hoidal M, et al. Multiplex gastrointestinal pathogens panels: implications for infection control. Diagn Microbiol Infect Dis. 2015 Jun;82(2):154-157.25796558
Riddle MS, DuPont HL, Connor BA. ACG Clinical Guidelines: Diagnosis, Treatment and Prevention of Acute Diarrheal Infections in Adults. Am J Gastroenterol. 2016 May;111(5):602-622.27068718
Safety Data Sheet (SDS/MSDS) FilmArray™ Reagent Kit.
Spina A, Kerr KG, Cormican M, et al. Spectrum of enteropathogens detected by FilmArray® GI Panel in a multicentre study of community-acquired gastroenteritis. Clin Microbiol Infect. 2015 Aug;21(8):719-728.25908431

Specimen Requirements

Specimen

Stool placed in a stool culture transport vial (Para-Pak® C&S orange)

Volume

Add stool until level in Para-Pak® vial is at fill-line on vial label. Do not over fill.

Container

Para-Pak® C&S Orange (Cary-Blair)

Collection Instructions

If possible, allow patient to urinate before collecting stool specimen so as to avoid contaminating the stool specimen with urine. Catch the stool specimen in a clean, empty wide-mouthed container or place plastic wrap over the opening of the toilet bowl to prevent the stool specimen from falling into the bowl. Do not mix urine or water with the stool specimen. For diaper collected specimens, line the diaper with plastic wrap. Do not submit the diaper. Place small amounts of the stool specimen into the orange-labeled Para-Pak® vial using the spoon affixed to the Para-Pak® vial cap, taking care not to let the volume in the vial exceed the RED fill-line indicated on the vial label. Cap the Para-Pak® vial and shake the vial 10 times to ensure complete distribution of the stool into the preservative. The stool specimen must be placed into the Para-Pak® vial within one hour of the stools production for optimum results. Label the Para-Pak® vial with the patient's name, date of birth, date of collection and time of collection. Be sure the Para-Pak® vial cap is securely in place and that the vial is not leaking.

Storage Instructions

Refrigerate. Room temperature storage and transport up to four days is acceptable.

Causes for Rejection

Specimen not received in Cary-Blair preservative medium; specimen vial leaking; specimen >96 hours since collection; frozen specimen; rectal swab; specimen below fill line on container (underfilled); container overfilled above line on label

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
183480 GI Profile, Stool, PCR 82195-9 183481 Campylobacter 82196-7
183480 GI Profile, Stool, PCR 82195-9 183482 C difficile toxin A/B 82197-5
183480 GI Profile, Stool, PCR 82195-9 183484 Plesiomonas shigelloides 82198-3
183480 GI Profile, Stool, PCR 82195-9 183485 Salmonella 82199-1
183480 GI Profile, Stool, PCR 82195-9 183486 Vibrio 82200-7
183480 GI Profile, Stool, PCR 82195-9 183487 Vibrio cholerae 82201-5
183480 GI Profile, Stool, PCR 82195-9 183488 Yersinia enterocolitica 82202-3
183480 GI Profile, Stool, PCR 82195-9 183489 Enteroaggregative E coli 80349-4
183480 GI Profile, Stool, PCR 82195-9 183490 Enteropathogenic E coli 80348-6
183480 GI Profile, Stool, PCR 82195-9 183492 Enterotoxigenic E coli 80351-0
183480 GI Profile, Stool, PCR 82195-9 183493 Shiga-toxin-producing E coli 82203-1
183480 GI Profile, Stool, PCR 82195-9 183494 E coli O157 82204-9
183480 GI Profile, Stool, PCR 82195-9 183495 Shigella/Enteroinvasive E coli 80350-2
183480 GI Profile, Stool, PCR 82195-9 183496 Cryptosporidium 82205-6
183480 GI Profile, Stool, PCR 82195-9 183497 Cyclospora cayetanensis 82206-4
183480 GI Profile, Stool, PCR 82195-9 183498 Entamoeba histolytica 82207-2
183480 GI Profile, Stool, PCR 82195-9 183500 Giardia lamblia 82208-0
183480 GI Profile, Stool, PCR 82195-9 183501 Adenovirus F 40/41 82209-8
183480 GI Profile, Stool, PCR 82195-9 183502 Astrovirus 82210-6
183480 GI Profile, Stool, PCR 82195-9 183503 Norovirus GI/GII 82211-4
183480 GI Profile, Stool, PCR 82195-9 183504 Rotavirus A 82212-2
183480 GI Profile, Stool, PCR 82195-9 183505 Sapovirus 82213-0
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183481
Result Code NameCampylobacter
UofM
Result LOINC82196-7
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183482
Result Code NameC difficile toxin A/B
UofM
Result LOINC82197-5
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183484
Result Code NamePlesiomonas shigelloides
UofM
Result LOINC82198-3
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183485
Result Code NameSalmonella
UofM
Result LOINC82199-1
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183486
Result Code NameVibrio
UofM
Result LOINC82200-7
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183487
Result Code NameVibrio cholerae
UofM
Result LOINC82201-5
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183488
Result Code NameYersinia enterocolitica
UofM
Result LOINC82202-3
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183489
Result Code NameEnteroaggregative E coli
UofM
Result LOINC80349-4
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183490
Result Code NameEnteropathogenic E coli
UofM
Result LOINC80348-6
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183492
Result Code NameEnterotoxigenic E coli
UofM
Result LOINC80351-0
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183493
Result Code NameShiga-toxin-producing E coli
UofM
Result LOINC82203-1
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183494
Result Code NameE coli O157
UofM
Result LOINC82204-9
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183495
Result Code NameShigella/Enteroinvasive E coli
UofM
Result LOINC80350-2
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183496
Result Code NameCryptosporidium
UofM
Result LOINC82205-6
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183497
Result Code NameCyclospora cayetanensis
UofM
Result LOINC82206-4
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183498
Result Code NameEntamoeba histolytica
UofM
Result LOINC82207-2
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183500
Result Code NameGiardia lamblia
UofM
Result LOINC82208-0
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183501
Result Code NameAdenovirus F 40/41
UofM
Result LOINC82209-8
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183502
Result Code NameAstrovirus
UofM
Result LOINC82210-6
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183503
Result Code NameNorovirus GI/GII
UofM
Result LOINC82211-4
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183504
Result Code NameRotavirus A
UofM
Result LOINC82212-2
Order Code183480
Order Code NameGI Profile, Stool, PCR
Order Loinc82195-9
Result Code183505
Result Code NameSapovirus
UofM
Result LOINC82213-0