Test Details
Methodology
Enzyme immunoassay (EIA)
Result Turnaround Time
1 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
West Nile virus, IgG; West Nile virus, IgM
Use
This test is indicated for use as an aid to the diagnosis of West Nile virus encephalitis. WNV IgM antibodies typically appear three to eight days after illness onset and persist 30 to 90 days (but can persist up to 500 days). WNV IgG antibodies appear shortly after IgM antibodies and persist greater than 500 days. Due to cross-reactivity with other flaviviruses, confirmation by neutralizing antibody assay may be recommended.
Limitations
The performance of this assay has not been established for screening the general population. Testing should only be performed on patients with clinical symptoms consistent with WNV encephalitis. Serological cross-reactivity across the flavivirus group is common (i.e., between Dengue virus, Japanese encephalitis virus, St. Louis encephalitis virus, yellow fever virus and others).
References
Specimen Requirements
Specimen
Cerebrospinal fluid (CSF)
Volume
0.5 mL
Minimum Volume
0.2 mL
Container
Sterile container
Collection Instructions
Do not centrifuge cerebrospinal fluid.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Reference Range
Negative
Storage Instructions
Room temperature
Causes for Rejection
Gross contamination; severe hemolysis; quantity not sufficient for analysis
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 138966 | West Nile Virus Antibody, CSF | 94853-9 | 139039 | West Nile Virus, IgG | 41236-1 | |
| 138966 | West Nile Virus Antibody, CSF | 94853-9 | 139043 | West Nile Virus, IgM | 31703-2 | |
| Order Code | 138966 | |||||
| Order Code Name | West Nile Virus Antibody, CSF | |||||
| Order Loinc | 94853-9 | |||||
| Result Code | 139039 | |||||
| Result Code Name | West Nile Virus, IgG | |||||
| UofM | ||||||
| Result LOINC | 41236-1 | |||||
| Order Code | 138966 | |||||
| Order Code Name | West Nile Virus Antibody, CSF | |||||
| Order Loinc | 94853-9 | |||||
| Result Code | 139043 | |||||
| Result Code Name | West Nile Virus, IgM | |||||
| UofM | ||||||
| Result LOINC | 31703-2 |