T-Cell Activation Profile, CD8 Subsets

TEST: 505750
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CPT: 86356(x3); 86359; 86360
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Test Includes

Percentage and absolute values CD3+, CD3+CD4+, CD3+CD8+, CD3+CD25+, CD8+CD38+, CD8+HLA-DR+, CD4:CD8 ratio; CBC with differential

Expected Turnaround Time

1 - 3 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Specimen Requirements

Specimen

Whole blood

Volume

Fill tube(s) to capacity.

Container

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube

Collection

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature

Period

Room temperature

Yellow-top (ACD-A) or (ACD-B) tube: 2 days

Lavender-top (EDTA) tube: 1 day

Refrigerated

Unstable

Frozen

Unstable

Freeze/thaw cycles

Unstable

Causes for Rejection

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

Test Details

Use

This test is an expanded profile for AIDS monitoring and prognosis. An increase in activation markers is associated with poor prognosis.

Limitations

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Methodology

Flow cytometry

Reference Interval

Reference intervals are established by the laboratory. See table.

Adult Reference Intervals for Lymphocyte Immunophenotyping

CD

95% Confidence Interval

Percentage

Cells/mm3

Minimum

Maximum

Minimum

Maximum

CD3+

57.5

86.2

622

2402

CD3+CD4+

30.8

58.5

359

1519

CD3+CD8+

12.0

35.5

109

897

CD3+CD25+

4.9

25.9

79

535

T4:T8 ratio

0.92

3.72

CD8+CD38+

0

17.7

0

381

CD8+HLA-DR+

0

4.9

0

117

References

Landay AL, Jessop C, Lennette ET, Levy JA. Chronic fatigue syndrome: Clinical condition associated with immune activation. Lancet. 1991 Sep 21; 338(8769):707-712. 1679864
Sadler D, Keren DF. Surface marker assays in immunodeficiency diseases. In: Keren DF, Hanson CA, Hurtubise PE, eds. Flow Cytometry and Clinical Diseases. Chicago, Ill: ASCP Press;1994:chap 9.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
505750 T-Cell Activation, CD8 Subsets 65758-5 505350 Absolute CD 3 /uL 8122-4
505750 T-Cell Activation, CD8 Subsets 65758-5 505324 % CD 3 Pos. Lymph. % 8124-0
505750 T-Cell Activation, CD8 Subsets 65758-5 505351 Absolute CD 4 Helper /uL 24467-3
505750 T-Cell Activation, CD8 Subsets 65758-5 505326 % CD 4 Pos. Lymph. % 8123-2
505750 T-Cell Activation, CD8 Subsets 65758-5 505352 Absolute CD 8 (Supp) /uL 14135-8
505750 T-Cell Activation, CD8 Subsets 65758-5 505328 % CD 8 Pos. Lymph. % 8101-8
505750 T-Cell Activation, CD8 Subsets 65758-5 505333 CD4/CD8 Ratio 54218-3
505750 T-Cell Activation, CD8 Subsets 65758-5 505162 Abs.CD8+HLA-DR+Lymph /uL 16107-5
505750 T-Cell Activation, CD8 Subsets 65758-5 505161 % CD8+HLA-DR+ Lymphs % 13337-1
505750 T-Cell Activation, CD8 Subsets 65758-5 505157 % CD3+CD25+ Lymphs % 13333-0
505750 T-Cell Activation, CD8 Subsets 65758-5 505158 Abs.CD3+CD25+ Lymphs /uL 26997-7
505750 T-Cell Activation, CD8 Subsets 65758-5 505151 % CD8+CD38+ Lymphs % 13344-7
505750 T-Cell Activation, CD8 Subsets 65758-5 505153 Abs.CD8+CD38+ Lymphs /uL 21374-4
505750 T-Cell Activation, CD8 Subsets 65758-5 005025 WBC x10E3/uL 6690-2
505750 T-Cell Activation, CD8 Subsets 65758-5 005033 RBC x10E6/uL 789-8
505750 T-Cell Activation, CD8 Subsets 65758-5 005041 Hemoglobin g/dL 718-7
505750 T-Cell Activation, CD8 Subsets 65758-5 005058 Hematocrit % 4544-3
505750 T-Cell Activation, CD8 Subsets 65758-5 015065 MCV fL 787-2
505750 T-Cell Activation, CD8 Subsets 65758-5 015073 MCH pg 785-6
505750 T-Cell Activation, CD8 Subsets 65758-5 015081 MCHC g/dL 786-4
505750 T-Cell Activation, CD8 Subsets 65758-5 105007 RDW % 788-0
505750 T-Cell Activation, CD8 Subsets 65758-5 015172 Platelets x10E3/uL 777-3
505750 T-Cell Activation, CD8 Subsets 65758-5 015107 Neutrophils % 770-8
505750 T-Cell Activation, CD8 Subsets 65758-5 015123 Lymphs % 736-9
505750 T-Cell Activation, CD8 Subsets 65758-5 015131 Monocytes % 5905-5
505750 T-Cell Activation, CD8 Subsets 65758-5 015149 Eos % 713-8
505750 T-Cell Activation, CD8 Subsets 65758-5 015156 Basos % 706-2
505750 T-Cell Activation, CD8 Subsets 65758-5 115398 Immature Cells N/A
505750 T-Cell Activation, CD8 Subsets 65758-5 015909 Neutrophils (Absolute) x10E3/uL 751-8
505750 T-Cell Activation, CD8 Subsets 65758-5 015917 Lymphs (Absolute) x10E3/uL 731-0
505750 T-Cell Activation, CD8 Subsets 65758-5 015925 Monocytes(Absolute) x10E3/uL 742-7
505750 T-Cell Activation, CD8 Subsets 65758-5 015933 Eos (Absolute) x10E3/uL 711-2
505750 T-Cell Activation, CD8 Subsets 65758-5 015941 Baso (Absolute) x10E3/uL 704-7
505750 T-Cell Activation, CD8 Subsets 65758-5 015108 Immature Granulocytes % 71695-1
505750 T-Cell Activation, CD8 Subsets 65758-5 015911 Immature Grans (Abs) x10E3/uL 53115-2
505750 T-Cell Activation, CD8 Subsets 65758-5 015945 NRBC % 58413-6
505750 T-Cell Activation, CD8 Subsets 65758-5 015180 Hematology Comments: 18314-5
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115400 Bands % 35332-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115401 Metamyelocytes % 28541-1
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115403 Myelocytes % 26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115404 Promyelocytes % 26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115405 Blasts/blast like cells % 26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115406 Megakaryocytes % 19252-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115265 Other, Lineage Uncertain % 55433-7

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