Test Details
Methodology
High-pressure liquid chromatography (HPLC)/tandem mass spectrometry
Result Turnaround Time
7 - 9 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.
Special Instructions
For pregnenolone without stimulation, order Pregnenolone, Mass Spectrometry (Endocrine Sciences) [140707].
Draw blood for baseline pregnenolone. Inject cosyntropin: 15 μg/kg in neonates; 125 μg in children younger than and including two-year-old children; and 250 μg in children older than two years and adults. IM or IV (If IV, dilute cosyntropin in 2 to 5 mL of sterile saline and inject over two minutes). Draw blood for stimulated pregnenolone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.
Specimen Requirements
Specimen
Serum (preferred) or plasma, frozen
Volume
1 mL (each specimen)
Minimum Volume
0.4 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube
Collection Instructions
Draw two samples: one baseline and one stimulated 60 minutes. If a tube other than a gel-barrier is used, transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same test request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Stability Requirements
Temperature | Period |
|---|---|
Refrigerated | 1 day |
Frozen | 2 years |
Storage Instructions
Freeze.
Causes for Rejection
Nonserum or non-EDTA/heparin plasma specimen
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 500773 | Pregnenolone, ACTH Stimulation | 500774 | Pregnenolone, Baseline | ng/dL | 2837-3 | |
| 500773 | Pregnenolone, ACTH Stimulation | 500775 | Pregnenolone, Stimulated | ng/dL | 46100-4 | |
| Order Code | 500773 | |||||
| Order Code Name | Pregnenolone, ACTH Stimulation | |||||
| Order Loinc | ||||||
| Result Code | 500774 | |||||
| Result Code Name | Pregnenolone, Baseline | |||||
| UofM | ng/dL | |||||
| Result LOINC | 2837-3 | |||||
| Order Code | 500773 | |||||
| Order Code Name | Pregnenolone, ACTH Stimulation | |||||
| Order Loinc | ||||||
| Result Code | 500775 | |||||
| Result Code Name | Pregnenolone, Stimulated | |||||
| UofM | ng/dL | |||||
| Result LOINC | 46100-4 |