Natural Killer Cell and Activated T-Cell Profile/IL-2r

TEST: 502500
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CPT: 86356; 86357; 86359
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Synonyms

  • CD25
  • Interleukin-2 Receptor
  • TAC Antigen

Test Includes

Percentage CD3+; absolute CD3+; percentage CD3+CD25+ (IL-2 receptor); absolute CD3+CD25+; percentage CD3-CD56+ (NK); total CD3-CD56+ (NK); CBC with differential; total lymphocyte count

Expected Turnaround Time

1 - 3 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Specimen Requirements

Specimen

Whole blood

Volume

Fill tube(s) to capacity.

Container

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube

Collection

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Storage Instructions

Maintain specimen at room temperature.

Stability Requirements

Temperature

Period

Room temperature

Yellow-top (ACD-A) or (ACD-B) tube: 2 days

Lavender-top (EDTA) tube: 1 day

Refrigerated

Unstable

Frozen

Unstable

Freeze/thaw cycles

Unstable

Causes for Rejection

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

Test Details

Use

This test is used to assess changes in T-lymphocyte surface markers that may indicate immune stimulation.

Limitations

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Methodology

Flow cytometry

Reference Interval

Reference intervals for adults have been established by the laboratory. See table.

Adult Reference Intervals for Lymphocyte Immunophenotyping

CD

95% Confidence Interval

Percentage

Cells/mm3

Minimum

Maximum

Minimum

Maximum

CD3+

58

86

622

2402

CD3+CD25+

5

26

79

535

CD3-CD56+

1

19

24

406

Additional Information

Natural killer (NK) cells are large granular lymphocytes that mediate MHC-unrestricted cytotoxicity against virus-infected and malignant cells and manufacture a number of cytokines following stimulation of the immune system.

The combination of IL-2 and T-cell markers can be used to assess the state of activation of T cells. NK cells are responsible for the destruction of virally infected cells, tumor cells, and antibody-dependent cellular cytotoxicity.

References

Caligiuri M, Murray C, Buchwald D, et al. Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. J Immunol. 1987 Nov 15; 139(10):3306-3313. 2824604
Landay AL, Jessop C, Lennette ET, Levy JA. Chronic fatigue syndrome: Clinical condition associated with immune activation. Lancet. 1991 Sep 21; 338(8769):707-712.1679864
Lanier L, Jackson A. Monoclonal antibodies: Differentiation antigens expressed on leukocytes. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 23.
Rich RR, ed. Clinical Immunology: Principles and Practice. St Louis, Mo: Mosby-Year Book;1996:chap 87.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
502500 NK and Activated T Cells/IL2r 502546 Ab NK (CD56/16) /uL 42188-3
502500 NK and Activated T Cells/IL2r 502545 % NK (CD56/16) % 42189-1
502500 NK and Activated T Cells/IL2r 505350 Absolute CD 3 /uL 8122-4
502500 NK and Activated T Cells/IL2r 505324 % CD 3 Pos. Lymph. % 8124-0
502500 NK and Activated T Cells/IL2r 505158 Abs.CD3+CD25+ Lymphs /uL 26997-7
502500 NK and Activated T Cells/IL2r 505157 % CD3+CD25+ Lymphs % 13333-0
502500 NK and Activated T Cells/IL2r 005025 WBC x10E3/uL 6690-2
502500 NK and Activated T Cells/IL2r 005033 RBC x10E6/uL 789-8
502500 NK and Activated T Cells/IL2r 005041 Hemoglobin g/dL 718-7
502500 NK and Activated T Cells/IL2r 005058 Hematocrit % 4544-3
502500 NK and Activated T Cells/IL2r 015065 MCV fL 787-2
502500 NK and Activated T Cells/IL2r 015073 MCH pg 785-6
502500 NK and Activated T Cells/IL2r 015081 MCHC g/dL 786-4
502500 NK and Activated T Cells/IL2r 105007 RDW % 788-0
502500 NK and Activated T Cells/IL2r 015172 Platelets x10E3/uL 777-3
502500 NK and Activated T Cells/IL2r 015107 Neutrophils % 770-8
502500 NK and Activated T Cells/IL2r 015123 Lymphs % 736-9
502500 NK and Activated T Cells/IL2r 015131 Monocytes % 5905-5
502500 NK and Activated T Cells/IL2r 015149 Eos % 713-8
502500 NK and Activated T Cells/IL2r 015156 Basos % 706-2
502500 NK and Activated T Cells/IL2r 115398 Immature Cells N/A
502500 NK and Activated T Cells/IL2r 015909 Neutrophils (Absolute) x10E3/uL 751-8
502500 NK and Activated T Cells/IL2r 015917 Lymphs (Absolute) x10E3/uL 731-0
502500 NK and Activated T Cells/IL2r 015925 Monocytes(Absolute) x10E3/uL 742-7
502500 NK and Activated T Cells/IL2r 015933 Eos (Absolute) x10E3/uL 711-2
502500 NK and Activated T Cells/IL2r 015941 Baso (Absolute) x10E3/uL 704-7
502500 NK and Activated T Cells/IL2r 015108 Immature Granulocytes % 71695-1
502500 NK and Activated T Cells/IL2r 015911 Immature Grans (Abs) x10E3/uL 53115-2
502500 NK and Activated T Cells/IL2r 015945 NRBC % 58413-6
502500 NK and Activated T Cells/IL2r 015180 Hematology Comments: 18314-5
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115400 Bands % 35332-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115401 Metamyelocytes % 28541-1
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115403 Myelocytes % 26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115404 Promyelocytes % 26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115405 Blasts/blast like cells % 26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115406 Megakaryocytes % 19252-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115265 Other, Lineage Uncertain % 55433-7

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