Myeloproliferative Neoplasms/Chronic Myelogenous Leukemia (MPN/CML), FISH

Test Number 511425

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CPT

Contact CPT coding department at 800-222-7566, ext 6-8400.

Synonyms

Leukemia/Lymphoma-specific FISH
Myeloproliferative Neoplasms

Test Details

Methodology

Fluorescence in situ hybridization (FISH)

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Oncology Fluorescence in situ Hybridization (FISH)

Test Includes

Fluorescence in situ hybridization (FISH) for myeloproliferative neoplasms/chronic myelogenous leukemia (MPN/CML) targeting numerical changes of chromosomes 8, 9, 13q14, and rearrangement of BCR/ABL1

Use

This test is used for confirmation/identification of chromosome abnormalities in interphase nuclei and leukemia monitoring of residual disease.

~~Confirmation/identification of chromosome abnormalities in interphase nuclei. L~~eukemia monitoring of residual disease.

This test is used for confirmation/identification of chromosome abnormalities in interphase nuclei and leukemia monitoring of residual disease.

Special Instructions

Include pertinent clinical diagnosis and previous cytogenetic studies on the test request form.

Include ~~the pertinent clinical diagnosis, previous cytogenetic studies, and probe of interest with~~ the test request form.

Include pertinent clinical diagnosis and previous cytogenetic studies on the test request form.

Limitations

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Specimen Requirements

Specimen

Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

~~Bone marrow, blood, cerebrospinal fluid (CSF), FNA, bone marrow touch prep slides~~

Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

Volume

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 10 slides with metaphase and/or interphase nuclei or 10 touch preparation slides

~~3 mL bone marrow (For additional cytogenetic studies an additional 1 mL of bone marrow is required.), fixed-cell pellet, three~~ slides

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 10 slides with metaphase and/or interphase nuclei or 10 touch preparation slides

Minimum Volume

1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 5 slides with metaphase and/or interphase nuclei or 5 touch preparation slides (Note: Minimum volumes may not allow for repeat testing.)

~~Fixed-cell pellet from a cytogenetic analysis, five slides, 2 mL blood, or 1 mL bone marrow (For additional cytogenetic studies, an additional 1 mL of bone marrow is required~~.)

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Green-top (~~heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube~~

Collection Instructions

Utilize sterile technique and Labcorp transport kits when possible.

Storage Instructions

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

~~Maintain specimens at room temperatur~~e.

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Causes for Rejection

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

Broken ~~or dirty slides; excessive cellular debris; stains on slide~~s