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Myelodysplastic Syndrome (MDS), FISH

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  • Updated on 03/31/2025

Test Details

Methodology

Fluorescence in situ Hybridization (FISH)

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

Fluorescence in situ hybridization (FISH) for myelodysplastic syndrome (MDS), targeting numerical changes associated with 5q, 7q, 8, and 20q.

Use

This test is a diagnostic test for myelodysplastic syndrome. The principal use is for interphase analysis of cases with no (or low) mitotic activity in cytogenetic analysis or interphase analysis from blood in cases of inaspirable bone marrow. Detection rate is approximately 80% of clones detected in cytogenetic analysis.

Diagnostic test for myelodysplastic syndrome. The principal use is for interphase analysis of cases with no (or low) mitotic activity in cytogenetic analysis or interphase analysis from blood in cases of inaspirable bone marrow. Detection rate is approximately 80% of clones detected in cytogenetic analysis.

This test is a diagnostic test for myelodysplastic syndrome. The principal use is for interphase analysis of cases with no (or low) mitotic activity in cytogenetic analysis or interphase analysis from blood in cases of inaspirable bone marrow. Detection rate is approximately 80% of clones detected in cytogenetic analysis.

Special Instructions

Indicate pertinent clinical diagnosis and previous cytogenetic studies on the test request form.

Limitations

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. 

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. 

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Specimen Requirements

Specimen

Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

 

Blood, bone marrow, fixed-cell pellet from a cytogenetic analysis, or slides prepared from a cytogenetic analysis

Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

 

Volume

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 8 slides with metaphase and/or interphase nuclei or 8 touch preparation slides

10 mL blood (adult), 3 to 5 mL blood (pediatric), 1 to 3 mL bone marrow, one fixed-cell pellet, or four slides

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 8 slides with metaphase and/or interphase nuclei or 8 touch preparation slides

Minimum Volume

1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 4 slides with metaphase and/or interphase nuclei or 4 touch preparation slides (Note: Minimum volumes may not allow for repeat testing.)

1 mL blood (adult/pediatric), 1 mL bone marrow, or four slides

1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 4 slides with metaphase and/or interphase nuclei or 4 touch preparation slides (Note: Minimum volumes may not allow for repeat testing.)

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavendar-top (EDTA) tube  (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Green-top (heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavendar-top (EDTA) tube  (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Collection Instructions

Utilize sterile technique and Labcorp transport kits when possible.

Utilize sterile technique and Labcorp transport kits when possible.

Storage Instructions

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Maintain specimen at room temperature.

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Causes for Rejection

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

Broken Vacutainer®; broken slides

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
511060 MDS FISH Panel 510616 Cells Counted 62361-1
511060 MDS FISH Panel 510617 Cells Analyzed 62360-3
511060 MDS FISH Panel 510618 FISH Result 57802-1
511060 MDS FISH Panel 511042 Interpretation 50398-7
511060 MDS FISH Panel 510620 Specimen Type 31208-2
511060 MDS FISH Panel 511038 Director Review: 48672-0
Order Code511060
Order Code NameMDS FISH Panel
Order Loinc
Result Code510616
Result Code NameCells Counted
UofM
Result LOINC62361-1
Order Code511060
Order Code NameMDS FISH Panel
Order Loinc
Result Code510617
Result Code NameCells Analyzed
UofM
Result LOINC62360-3
Order Code511060
Order Code NameMDS FISH Panel
Order Loinc
Result Code510618
Result Code NameFISH Result
UofM
Result LOINC57802-1
Order Code511060
Order Code NameMDS FISH Panel
Order Loinc
Result Code511042
Result Code NameInterpretation
UofM
Result LOINC50398-7
Order Code511060
Order Code NameMDS FISH Panel
Order Loinc
Result Code510620
Result Code NameSpecimen Type
UofM
Result LOINC31208-2
Order Code511060
Order Code NameMDS FISH Panel
Order Loinc
Result Code511038
Result Code NameDirector Review:
UofM
Result LOINC48672-0