Test Details
Methodology
Fluorescence in situ hybridization (FISH)
Result Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test is used for confirmation/identification of leukemia/lymphoma-related alterations and leukemia/lymphoma monitoring of residual disease, transplants and indolent clones (see list or call lab for probe availability).
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This test is used for confirmation/identification of leukemia/lymphoma-related alterations and leukemia/lymphoma monitoring of residual disease, transplants and indolent clones (see list or call lab for probe availability). |
Special Instructions
Include pertinent clinical diagnosis and previous cytogenetic studies on the test request form.
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Include pertinent clinical diagnosis and previous cytogenetic studies on the test request form. |
Limitations
Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.
This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration. |
Specimen Requirements
Specimen
Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei, bone marrow touch preparation slides, paraffin slides or block are acceptable for testing.
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Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei, bone marrow touch preparation slides, paraffin slides or block are acceptable for testing. |
Volume
5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 3 slides with metaphase and/or interphase nuclei, 3 touch preparation slides or 4 slides from paraffin block
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5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 3 slides with metaphase and/or interphase nuclei, 3 touch preparation slides or 4 slides from paraffin block |
Minimum Volume
1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 1 slide with metaphase and/or interphase nuclei, 1 touch preparation slide or 2 paraffin slides (Note: Minimum volumes may not allow for repeat testing.)
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1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 1 slide with metaphase and/or interphase nuclei, 1 touch preparation slide or 2 paraffin slides (Note: Minimum volumes may not allow for repeat testing.) |
Container
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube |
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity. |
Collection Instructions
Utilize sterile technique and Labcorp transport kits when possible.
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Utilize sterile technique and Labcorp transport kits when possible. |
Storage Instructions
Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.
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Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze. |
Causes for Rejection
Broken or dirty slides; excessive cellular debris or stained slides; decalcified bone cores; frozen specimen
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 510945 | MYCN Oncology FISH | 510290 | Specimen Type | 31208-2 | ||
| 510945 | MYCN Oncology FISH | 510291 | Cells Counted | 62361-1 | ||
| 510945 | MYCN Oncology FISH | 510294 | Cells Analyzed | 62360-3 | ||
| 510945 | MYCN Oncology FISH | 510946 | FISH Result | 48674-6 | ||
| 510945 | MYCN Oncology FISH | 510296 | Interpretation | 50398-7 | ||
| 510945 | MYCN Oncology FISH | 510947 | Director Review: | 48672-0 | ||
| Order Code | 510945 | |||||
| Order Code Name | MYCN Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510290 | |||||
| Result Code Name | Specimen Type | |||||
| UofM | ||||||
| Result LOINC | 31208-2 | |||||
| Order Code | 510945 | |||||
| Order Code Name | MYCN Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510291 | |||||
| Result Code Name | Cells Counted | |||||
| UofM | ||||||
| Result LOINC | 62361-1 | |||||
| Order Code | 510945 | |||||
| Order Code Name | MYCN Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510294 | |||||
| Result Code Name | Cells Analyzed | |||||
| UofM | ||||||
| Result LOINC | 62360-3 | |||||
| Order Code | 510945 | |||||
| Order Code Name | MYCN Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510946 | |||||
| Result Code Name | FISH Result | |||||
| UofM | ||||||
| Result LOINC | 48674-6 | |||||
| Order Code | 510945 | |||||
| Order Code Name | MYCN Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510296 | |||||
| Result Code Name | Interpretation | |||||
| UofM | ||||||
| Result LOINC | 50398-7 | |||||
| Order Code | 510945 | |||||
| Order Code Name | MYCN Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510947 | |||||
| Result Code Name | Director Review: | |||||
| UofM | ||||||
| Result LOINC | 48672-0 |