Test Details
Methodology
Line blot
Result Turnaround Time
1 - 5 days
Use
Detect antibodies specific for B burgdorferi. This test is intended as the second step in testing serum samples found to have been equivocal or positive using an ELISA or IFA test as the first step to provide supportive evidence of infection with B burgdorferi.
Special Instructions
This test is intended as the second step in testing serum samples found to have been equivocal or positive using an ELISA or IFA test as the first step to provide supportive evidence of infection with B burgdorferi. For CSF, order test 160457.
Specimen Requirements
Test Includes
Line blot analysis and interpretations for IgG- and IgM-specific antibodies
Custom Additional Information
Provides both IgM and IgG Line blot results. Increased specificity reduces cross-reactivity from other spirochetes. Detect and analyze antibodies against specific B burgdorferi proteins. The sensitivity of a Line blot assay by itself is not sufficient to assess Lyme infection adequately. Both EIA and Line blot tests should be performed.
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL
Container
Red-top tube or gel-barrier tube or serum transfer tube
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Hemolysis; lipemia; gross bacterial contamination
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Reference Range
Negative
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163640 | Lyme IgG Line Blot Interp. | 13502-0 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163635 | IgG P93 Ab. | 9597-6 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163605 | IgG P66 Ab. | 9596-8 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163623 | IgG P58 Ab. | 9595-0 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163641 | IgG P45 Ab. | 9594-3 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163607 | IgG P41 Ab. | 9593-5 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163598 | IgG P39 Ab. | 9592-7 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163633 | IgG P30 Ab. | 9591-9 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163636 | IgG P28 Ab. | 9590-1 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163642 | IgG P23 Ab. | 9589-3 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163627 | IgG P18 Ab. | 9588-5 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163639 | Lyme IgM Line Blot Interp. | 13503-8 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163616 | IgM P41 Ab. | 9587-7 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163599 | IgM P39 Ab. | 9599-2 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163638 | IgM P23 Ab. | 9598-4 | |
| 163600 | Lyme, Line Blot, Serum | 60342-3 | 163052 | Additional Information: | N/A | |
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163640 | |||||
| Result Code Name | Lyme IgG Line Blot Interp. | |||||
| UofM | ||||||
| Result LOINC | 13502-0 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163635 | |||||
| Result Code Name | IgG P93 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9597-6 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163605 | |||||
| Result Code Name | IgG P66 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9596-8 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163623 | |||||
| Result Code Name | IgG P58 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9595-0 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163641 | |||||
| Result Code Name | IgG P45 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9594-3 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163607 | |||||
| Result Code Name | IgG P41 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9593-5 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163598 | |||||
| Result Code Name | IgG P39 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9592-7 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163633 | |||||
| Result Code Name | IgG P30 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9591-9 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163636 | |||||
| Result Code Name | IgG P28 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9590-1 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163642 | |||||
| Result Code Name | IgG P23 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9589-3 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163627 | |||||
| Result Code Name | IgG P18 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9588-5 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163639 | |||||
| Result Code Name | Lyme IgM Line Blot Interp. | |||||
| UofM | ||||||
| Result LOINC | 13503-8 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163616 | |||||
| Result Code Name | IgM P41 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9587-7 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163599 | |||||
| Result Code Name | IgM P39 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9599-2 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163638 | |||||
| Result Code Name | IgM P23 Ab. | |||||
| UofM | ||||||
| Result LOINC | 9598-4 | |||||
| Order Code | 163600 | |||||
| Order Code Name | Lyme, Line Blot, Serum | |||||
| Order Loinc | 60342-3 | |||||
| Result Code | 163052 | |||||
| Result Code Name | Additional Information: | |||||
| UofM | ||||||
| Result LOINC | N/A |