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For hours, walk-ins and appointments.Hepatitis C Virus (HCV), Quantitative, RNA (Abbott® RealTime)
CPT:
87522
Synonyms
- Viral Load, Abbott, Real-time
Expected Turnaround Time
3 - 7 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
Specimen Requirements
Specimen
Serum or plasma
Volume
2 mL
Minimum Volume
1 mL (Note: This volume does not allow for repeat testing.)
Container
Collect specimen in red-top, serum separator (SST), lavender-top (EDTA), PPT™ or yellow-top (ACD-A) tubes. Ship in a screw-cap polypropylene frozen transport tube, centrifuged serum separator tube (SST) or centrifuged PPT™ tubes.
Collection
Collect specimen in red-top, serum separator (SST), lavender-top (EDTA), PPT™ or yellow-top (ACD-A) tubes. Centrifuge specimen. If drawn in a red-top, EDTA or ACD, the serum or plasma must be transferred to a screw-cap polypropylene transport tube and frozen. Whole-blood specimen may be held at room temperature for four hours or refrigerated for 72 hours prior to centrifugation. Serum and plasma specimens may be stored at room temperature for 20 hours, refrigerated 72 hours, frozen at -20°C for 60 days or -70°C long term. Ship frozen on dry ice. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 20 hours (stability provided by manufacturer or literature reference) |
Refrigerated | 72 hours (stability provided by manufacturer or literature reference) Combined room temperature and refrigerated storage times must not exceed 72 hours. |
Frozen | -20°C up to 60 days (stability provided by manufacturer or literature reference) -70°C long term (stability provided by manufacturer or literature reference) |
Freeze/thaw cycles | Stable x0 |
Causes for Rejection
Hemolysis; plasma other than EDTA or ACD-A; specimen received in a "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; whole blood; frozen glass tubes
Test Details
Use
This test is used to determine the number of international units (IU) of hepatitis C virus (HCV) RNA per milliliter of serum or plasma in known HCV-positive patients.
Limitations
Assay range: 12 IU/mL to 100,000,000 IU/mL
Methodology
Abbott Alinity m HCV assay
References
Huang S, Salituro J, Tang N, et al. Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR. Nucleic Acids Res. 2007; 35(16):e101. 17693434
Tang N, Huang S, Salituro J, et al. A realtime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A-H, group O and group N samples. J Virol Methods. 2007 Dec; 146(1-2):236-245. 17707519
Vermehren J, Yu ML, Monto A, et al. Multi-center evaluation of the Abbott RealTime HCV assay for monitoring patients undergoing antiviral therapy for chronic hepatitis C. J Clin Virol. 2011 Oct; 52(2):133-137. 21803650
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 551300 | HCV RealTime Abbott | 11011-4 | 551301 | Hepatitis C Quantitation | IU/mL | 11011-4 |
| 551300 | HCV RealTime Abbott | 11011-4 | 551302 | HCV log10 | log10 IU/mL | 38180-6 |
| 551300 | HCV RealTime Abbott | 11011-4 | 551304 | Test Information: | N/A |
| Reflex Table for Hepatitis C Quantitation | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551305 | HCV RNA (International Units) | 551306 | HCV RNA (International Units) | IU/mL | 11011-4 |
| Reflex Table for Hepatitis C Quantitation | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551305 | HCV RNA (International Units) | 551307 | HCV log10 | log10 IU/mL | 38180-6 |
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