Test Details
Methodology
Quantitative test: cobas® HBV for use on the cobas® 6800/8800 system
Genotype test (reflex): Polymerase chain reaction (PCR) amplification and DNA sequencing
Result Turnaround Time
7 - 9 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test quantitates HBV DNA in serum or plasma with reflex to HBV genotype if ≥500 IU/mL is detected.
Limitations
The quantitative test has a range of 10 IU/mL to 1,000,000,000 IU/mL. The genotype assay is only triggered when the quantitative test result is ≥500 IU/mL.
Specimen Requirements
Specimen
Plasma or serum, frozen
Volume
3 mL
Minimum Volume
1.5 mL (Note: This volume does not allow for repeat testing.)
1.5 mL |
1.5 mL (Note: This volume does not allow for repeat testing.) |
Container
Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or PPT. Do not use green-top (heparin) or yellow-top (ACD) tube
Collection Instructions
Centrifuge sample within six hours of collection. Transfer the serum/plasma to a screw-top polypropylene tube and freeze.
Storage Instructions
Freeze.
Causes for Rejection
Green-top (heparin) or yellow-top (ACD) tubes used for collection; serum/plasma not frozen; PPT not centrifuged
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 551722 | HBV Quant PCR Rfx to Genotype | 42595-9 | 551651 | HBV IU/mL | IU/mL | 42595-9 |
| 551722 | HBV Quant PCR Rfx to Genotype | 42595-9 | 551585 | log10 HBV as IU/mL | log10 IU/mL | 48398-2 |
| 551722 | HBV Quant PCR Rfx to Genotype | 42595-9 | 551724 | Test Information: | 19147-8 | |
| 551722 | HBV Quant PCR Rfx to Genotype | 42595-9 | 551726 | HBV Genotype | N/A | |
| Order Code | 551722 | |||||
| Order Code Name | HBV Quant PCR Rfx to Genotype | |||||
| Order Loinc | 42595-9 | |||||
| Result Code | 551651 | |||||
| Result Code Name | HBV IU/mL | |||||
| UofM | IU/mL | |||||
| Result LOINC | 42595-9 | |||||
| Order Code | 551722 | |||||
| Order Code Name | HBV Quant PCR Rfx to Genotype | |||||
| Order Loinc | 42595-9 | |||||
| Result Code | 551585 | |||||
| Result Code Name | log10 HBV as IU/mL | |||||
| UofM | log10 IU/mL | |||||
| Result LOINC | 48398-2 | |||||
| Order Code | 551722 | |||||
| Order Code Name | HBV Quant PCR Rfx to Genotype | |||||
| Order Loinc | 42595-9 | |||||
| Result Code | 551724 | |||||
| Result Code Name | Test Information: | |||||
| UofM | ||||||
| Result LOINC | 19147-8 | |||||
| Order Code | 551722 | |||||
| Order Code Name | HBV Quant PCR Rfx to Genotype | |||||
| Order Loinc | 42595-9 | |||||
| Result Code | 551726 | |||||
| Result Code Name | HBV Genotype | |||||
| UofM | ||||||
| Result LOINC | N/A |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 551587 | HBV Real-Time PCR, Quant | 551649 | HBV IU/mL | IU/mL | 42595-9 |
| Reflex 1 | ||||||
| Order Code | 551587 | |||||
| Order Name | HBV Real-Time PCR, Quant | |||||
| Result Code | 551649 | |||||
| Result Name | HBV IU/mL | |||||
| UofM | IU/mL | |||||
| Result LOINC | 42595-9 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 551587 | HBV Real-Time PCR, Quant | 551586 | log10 HBV IU/mL | log10 IU/mL | 48398-2 |
| Reflex 1 | ||||||
| Order Code | 551587 | |||||
| Order Name | HBV Real-Time PCR, Quant | |||||
| Result Code | 551586 | |||||
| Result Name | log10 HBV IU/mL | |||||
| UofM | log10 IU/mL | |||||
| Result LOINC | 48398-2 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 551727 | HBV Genotype | 551578 | HBV Genotype | 32366-7 | |
| Reflex 1 | ||||||
| Order Code | 551727 | |||||
| Order Name | HBV Genotype | |||||
| Result Code | 551578 | |||||
| Result Name | HBV Genotype | |||||
| UofM | ||||||
| Result LOINC | 32366-7 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 551727 | HBV Genotype | 551714 | HBV PreCore Mutation | 54210-0 | |
| Reflex 1 | ||||||
| Order Code | 551727 | |||||
| Order Name | HBV Genotype | |||||
| Result Code | 551714 | |||||
| Result Name | HBV PreCore Mutation | |||||
| UofM | ||||||
| Result LOINC | 54210-0 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 551727 | HBV Genotype | 551763 | HBV Genotype/PreCore | N/A | |
| Reflex 1 | ||||||
| Order Code | 551727 | |||||
| Order Name | HBV Genotype | |||||
| Result Code | 551763 | |||||
| Result Name | HBV Genotype/PreCore | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||