FKHR Oncology FISH

Test Number 510371

Test number copied

CPT

Contact CPT coding department at 800-222-7566, ext 6-8400.

Synonyms

Leukemia FISH
Lymphoma FISH

Test Details

Methodology

Fluorescence in situ hybridization (FISH)

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Fluorescence in situ Hybridization (FISH), Oncology [510669]

>Fluorescence in situ Hybridization (FISH), Oncology
Oncology Fluorescence in situ Hybridization (FISH)

href="https://www.labcorp.com/tests/510669/fluorescence-in-situ-hybridization-fish-oncology">Fluorescence in situ Hybridization (FISH), Oncology [510669]

Use

This test is used for confirmation/identification of cancer-related alterations (alveolar rhabdomyosarcoma).

~~Confirmation/identification of cancer-related alterations (alveolar rhabdomyosarcoma)~~

This test is used for confirmation/identification of cancer-related alterations (alveolar rhabdomyosarcoma).

Special Instructions

There will be an additional charge for processing paraffin block samples or for samples that require culture.

Limitations

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Custom Additional Information

A FKHR gene rearrangement is found in alveolar rhabdomyosarcoma (A-RMS). A-RMS is characterized by two pathognomonic translocations which generate gene fusions: t(2;13)(q35;q14) [PAX3-FKHR] and t(1;13)(p36;q14) [PAX7-FKHR], found in 80% and 15% of cases, respectively.

Specimen Requirements

Specimen

Fixed-cell pellet from a cytogenetic analysis, slides with metaphases and/or interphase nuclei or bone marrow touch prep slides; whole blood, bone marrow, cerebrospinal fluid (CSF), FNA, paraffin block are also acceptable with additional charge

Fixed-cell pellet from a cytogenetic analysis, slides with metaphases and/or interphase nuclei~~, or bone marrow touch prep slides. Blood, bone marrow, cerebrospinal fluid (CSF), FNA, paraffin block are also acceptable with additional charge.~~

Volume

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 2 slides with metaphase and/or interphase nuclei, 2 touch preparation slides or 3 slides from paraffin block

~~Fixed-cell pellet, three slides, 10 mL blood (adult), 5 mL blood (pediatric), or 3 mL bone marrow~~

Minimum Volume

1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 1 slide with metaphase and/or interphase nuclei, 1 touch preparation slide or 2 paraffin slides (Note: Minimum volumes may not allow for repeat testing.)

~~Fixed-cell pellet from a cytogenetic analysis, two slides, 5 mL blood (adult), 1 mL blood (pediatric), or 1 mL bone marrow~~

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

~~Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube~~

Collection Instructions

Utilize sterile technique and Labcorp transport kits when possible.

~~Transport to the testing lab at room temperature; use a cool pack or LabCorp transport kit. Do not allow sample to freeze. Sample must arrive in the laboratory within 72 hours of collection~~.

Utilize sterile technique and Labcorp transport kits when possible.

Storage Instructions

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

~~Maintain specimen at room temperatur~~e.