Test Details
Methodology
Fluorescence in situ hybridization (FISH)
Result Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test is used for confirmation/identification of cancer-related alterations (Ewing sarcoma).
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This test is used for confirmation/identification of cancer-related alterations (Ewing sarcoma). |
Special Instructions
There will be an additional charge for processing paraffin block samples or for samples that require culture.
Limitations
Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.
This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration. |
Custom Additional Information
Ewing sarcoma EWSR1 rearrangement (usually fused with FLI1) is the primary alteration in PNETs and also in desmoplastic round cell tumors where it is fused with WT1. EWSR1 is also rearranged in clear cell and chondrosarcomas.
Specimen Requirements
Specimen
Fixed-cell pellet from a cytogenetic analysis, slides with metaphases and/or interphase nuclei or bone marrow touch prep slides; whole blood, bone marrow, cerebrospinal fluid (CSF), FNA, paraffin block are also acceptable with additional charge
Fixed-cell pellet from a cytogenetic analysis, slides with metaphases and/or interphase nuclei |
Fixed-cell pellet from a cytogenetic analysis, slides with metaphases and/or interphase nuclei or bone marrow touch prep slides; whole blood, bone marrow, cerebrospinal fluid (CSF), FNA, paraffin block are also acceptable with additional charge |
Volume
5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 2 slides with metaphase and/or interphase nuclei, 2 touch preparation slides or 3 slides from paraffin block
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5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 2 slides with metaphase and/or interphase nuclei, 2 touch preparation slides or 3 slides from paraffin block |
Minimum Volume
1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 1 slide with metaphase and/or interphase nuclei, 1 touch preparation slide or 2 paraffin slides (Note: Minimum volumes may not allow for repeat testing.)
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1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 1 slide with metaphase and/or interphase nuclei, 1 touch preparation slide or 2 paraffin slides (Note: Minimum volumes may not allow for repeat testing.) |
Container
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube |
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity. |
Collection Instructions
Utilize sterile technique and Labcorp transport kits when possible.
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Utilize sterile technique and Labcorp transport kits when possible. |
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Broken or dirty slides; excessive cellular debris or stained slides; decalcified bone cores; frozen specimen
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 510379 | EWSR1 Oncology FISH | 510280 | Specimen Type | 31208-2 | ||
| 510379 | EWSR1 Oncology FISH | 510281 | Cells Counted | N/A | ||
| 510379 | EWSR1 Oncology FISH | 510284 | Cells Analyzed | N/A | ||
| 510379 | EWSR1 Oncology FISH | 510377 | FISH Result | 48674-6 | ||
| 510379 | EWSR1 Oncology FISH | 510286 | Interpretation | 50398-7 | ||
| 510379 | EWSR1 Oncology FISH | 510378 | Director Review: | 48672-0 | ||
| Order Code | 510379 | |||||
| Order Code Name | EWSR1 Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510280 | |||||
| Result Code Name | Specimen Type | |||||
| UofM | ||||||
| Result LOINC | 31208-2 | |||||
| Order Code | 510379 | |||||
| Order Code Name | EWSR1 Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510281 | |||||
| Result Code Name | Cells Counted | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 510379 | |||||
| Order Code Name | EWSR1 Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510284 | |||||
| Result Code Name | Cells Analyzed | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 510379 | |||||
| Order Code Name | EWSR1 Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510377 | |||||
| Result Code Name | FISH Result | |||||
| UofM | ||||||
| Result LOINC | 48674-6 | |||||
| Order Code | 510379 | |||||
| Order Code Name | EWSR1 Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510286 | |||||
| Result Code Name | Interpretation | |||||
| UofM | ||||||
| Result LOINC | 50398-7 | |||||
| Order Code | 510379 | |||||
| Order Code Name | EWSR1 Oncology FISH | |||||
| Order Loinc | ||||||
| Result Code | 510378 | |||||
| Result Code Name | Director Review: | |||||
| UofM | ||||||
| Result LOINC | 48672-0 |