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For hours, walk-ins and appointments.Synonyms
- DAT
- Direct Antihuman Globulin Test
- Direct Coombs'
Test Includes
Direct antiglobulin test with polyspecific antihuman globulin serum. Red cells found to be positive are further tested with monospecific antisera (anti-IgG and anticomplement).
Expected Turnaround Time
1 - 2 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Whole blood
Volume
4 mL
Minimum Volume
0.5 mL
Container
Lavender-top (EDTA) tube
Storage Instructions
Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze.
Causes for Rejection
Gross hemolysis; bacterial or other contamination; specimen more than five days old
Test Details
Use
Detect in vivo coating of IgG and/or complement components on RBCs
Limitations
The presence of potent cold autoagglutinins may interfere with test interpretation. Testing performed using clotted blood specimens may yield false-positive results with anticomplement.
Methodology
Antiglobulin test
Reference Interval
Negative
Additional Information
Positive direct antiglobulin tests are associated with a variety of conditions which include hemolytic transfusion reaction, autoimmune hemolytic anemia, hemolytic disease of the fetus/newborn, and may be secondary to certain pathological conditions or drug therapy. Note: The presence of a positive direct antiglobulin test does not necessarily correlate with a pathological condition.
References
Fung MK, Grossman JB, Hillyer CD, Westoff CM, eds. AABB Technical Manual. 18th ed. Bethesda, Md: American Association of Blood Banks;2014.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 006270 | Coombs', Direct | 1007-4 | 006270 | Coombs', Direct | 1007-4 |
| Reflex Table for Coombs', Direct | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 006271 | Anti-Complement+Anti-IgG | 006272 | Anti-IgG | 1006-6 | |
| Reflex Table for Coombs', Direct | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 006271 | Anti-Complement+Anti-IgG | 006273 | Anti-Complement | 13946-9 | |
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