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- vWF Collagen Binding Activity
Test Includes
Collagen-binding ratio; vWF antigen; vWF collagen-binding activity
Expected Turnaround Time
3 - 9 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
For more information, please view the literature below.
Procedures for Hemostasis and Thrombosis: A Clinical Test Compendium
Specimen Requirements
Specimen
Plasma, frozen
Volume
2 mL
Container
Blue-top (sodium citrate) tube
Collection
Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples except when using a winged blood collection device (ie, "butterfly"), in which case a discard tube should be used.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a Labcorp PP transpak frozen purple tube with screw cap (Labcorp No. 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume.
Storage Instructions
Freeze; four freeze/thaw cycles are acceptable. Stable refrigerated for four hours.
Causes for Rejection
Lipemia; icteric specimen; hemolysis; clotted specimen; specimen contaminated with heparin (ie, drawn with blood gases)
Test Details
Use
Screen for the presence of type II von Willebrand disease in plasma6
Limitations
vWF is an acute phase reactant protein and levels may be increased transiently into the normal range in some individuals with mild vWD following adrenergic stimulation or in response to certain drug therapies such as DDAVP or estrogen.
This procedure may be considered by Medicare and other carriers as investigational and, therefore, may not be payable as a covered benefit for patients.
Methodology
Enzyme-linked immunosorbent assay (ELISA)
Reference Interval
• CBA activity: 50% to 150%
• CBA ratio: 0.6−5.0
• vWF antigen: 50% to 150%
Additional Information
The collagen binding assay (CBA) can aid in the differentiation between von Willebrand disease type I and type II when used in conjunction with the vWF antigen assay. This assay evaluates the ability of high molecular weight multimers present in the patient's sample to bind collagen which is coated on the microtiter plates. The amount of vWF bound is measured using peroxidase-conjugated antibodies to human vWF. Following the addition of substrate, color intensity is proportional to the high molecular weight multimers present in the sample. Results are expressed as percent collagen-binding activity and also as a ratio of percent collagen-binding activity to total vWF antigen. The CBA activity correlates more closely with vWF function and bleeding problems than ELISA for vWF antigen levels.
Footnotes
1. Adcock DM, Kressin DC, Marlar RA. Effect of 3.2% vs 3.8% sodium citrate concentration on routine coagulation testing. Am J Clin Pathol. 1997 Jan; 107(1):105-110. 8980376
2. Reneke J, Etzell J, Leslie S, Ng VL, Gottfried EL. Prolonged prothrombin time and activated partial thromboplastin time due to underfilled specimen tubes with 109 mmol/L (3.2%) citrate anticoagulant. Am J Clin Pathol. 1998 Jun; 109(6):754-757. 9620035
3. National Committee for Clinical Laboratory Standardization. Collection, Transport, and Processing of Blood Specimens for Coagulation Testing and General Performance of Coagulation Assays; Approved Guideline. 5th ed. Villanova, Pa: NCCLS; 2008. Document H21-A5:28(5).
4. Gottfried EL, Adachi MM. Prothrombin time and activated partial thromboplastin time can be performed on the first tube. Am J Clin Pathol. 1997 Jun; 107(6):681-683. 9169665
5. McGlasson DL, More L, Best HA, Norris WL, Doe RH, Ray H. Drawing specimens for coagulation testing: Is a second tube necessary? Clin Lab Sci. 1999 May-Jun; 12(3):137-139. 10539100
6. Brandt JT, Triplett DA, Alving B, Scharrer I. Criteria for the diagnosis of lupus anticoagulants: An update. On behalf of the Subcommittee on Lupus Anticoagulant/Antiphospholipid Antibody of the Scientific and Standardisation Committee of the ISTH. Thromb Haemost. 1995 Oct; 74(4):1185-1190. 8560433
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 500360 | Collagen Binding Assay | 500357 | von Willebrand Factor Antigen | % | 27816-8 | |
| 500360 | Collagen Binding Assay | 500552 | vWF Collagen Binding Act. ** | % | 50377-1 | |
| 500360 | Collagen Binding Assay | 500359 | vWF Collagen Binding | ratio | 50378-9 |
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