Test Details
Methodology
Short-term cell culture; synchronization; chromosome harvest; G-banding; analysis (20 metaphases) and karyotyping; long-term β-mitogen cultures in some β-cell leukemias. Reflex to Reveal® microarray if chromosome analysis is normal. SNP microarray analysis is performed using the Cytoscan® HD platform, which uses more than 743,000 SNP probes and 1,953,000 NPCN probes with a median spacing of 0.88 kb.
Result Turnaround Time
7 - 10 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Short- and (in some cases) long-term β-mitogen culturing of specimens, chromosome preparation, and analysis of 20 metaphases; multiple karyotypes prepared, including all abnormal clones; reflex to SNP microarray (Reveal®) if chromosome analysis is normal
Use
Karyotyping, physical localization of copy number changes, and mapping of breakpoints involved in translocations for the diagnosis/prognosis of leukemia/lymphoma subgroups. The SNP assay will detect chromosomal imbalance that may be present in neoplastic disorders and clonal evolution.It provides detection of copy-neutral loss of heterozygosity of any chromosome, and the percent and location of homozygosity, that may be associated with cancer gene mutations.
Special Instructions
Indicate the tentative diagnosis on the test request form. This is necessary for proper culturing and result interpretation. Please direct any questions regarding this test to customer service at 800-345-4363.
Limitations
In studies of myeloid malignancy (eg, MDS in blood), availability of immature blasts, excessive amounts of sodium heparin, or use of lithium heparin, or EDTA will inhibit growth of cell culture. The SNP assay does not detect balanced rearrangements, low-level mosaicism (<10%), marker chromosomes that only contain heterochromatin or tetraploidy.
This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.
Specimen Requirements
Specimen
Bone marrow, blood (>4% blasts required; see Collection), lymph node, spleen, bone core, or effusion
Volume
2 mL bone marrow, 3 mL peripheral blood, or 0.5 to 1 cm³ tissue
Container
Bone marrow or peripheral blood in pediatric green-stopper (sodium heparin) tube; lymph node, spleen, bone core, or effusion in lymph node transport bottle
Collection Instructions
The requirement of >4% blasts for blood does not apply to cases of CLL, hairy cell leukemia, and some cases of small cell lymphocytic leukemia (SCLL) or lymphoma, which can be β-mitogen stimulated. Submit bone marrow or peripheral blood at room temperature using the Leukemia/Lymphoma Specimen Transport Kit (supplied by LabCorp). Submit lymph node, spleen, or bone core at room temperature using Lymph Node Transport Kit (supplied by LabCorp). Effusions may be sent in the same sterile containers. Specimens should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on the test request form.
Storage Instructions
Maintain specimen at room temperature.
Patient Preparation
Patient should not be on chemotherapy.
Causes for Rejection
Hemolysis; gross contamination; nonneoplastic cells; clotted specimen; frozen specimen; fixed specimen; use of improper or too much anticoagulant. For SNP microarray, quantity not sufficient for analysis, gel-separator tubes, microtainer tubes, fixed cell pellet.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 511040 | Chromo Leuk/Lymph Rfx CMA | 510678 | Specimen Type | 31208-2 | ||
| 511040 | Chromo Leuk/Lymph Rfx CMA | 510679 | Cells Counted | 62361-1 | ||
| 511040 | Chromo Leuk/Lymph Rfx CMA | 510680 | Cells Analyzed | 62360-3 | ||
| 511040 | Chromo Leuk/Lymph Rfx CMA | 510681 | Cells Karyotyped | 55199-4 | ||
| 511040 | Chromo Leuk/Lymph Rfx CMA | 510682 | GTG Band Resolution Achieved | 62358-7 | ||
| 511040 | Chromo Leuk/Lymph Rfx CMA | 510683 | Cytogenetic Result | 62356-1 | ||
| 511040 | Chromo Leuk/Lymph Rfx CMA | 511029 | Interpretation | 62365-2 | ||
| 511040 | Chromo Leuk/Lymph Rfx CMA | 511032 | Director Review: | 48672-0 | ||
| 511040 | Chromo Leuk/Lymph Rfx CMA | 052323 | Reflex | N/A | ||
| Order Code | 511040 | |||||
| Order Code Name | Chromo Leuk/Lymph Rfx CMA | |||||
| Order Loinc | ||||||
| Result Code | 510678 | |||||
| Result Code Name | Specimen Type | |||||
| UofM | ||||||
| Result LOINC | 31208-2 | |||||
| Order Code | 511040 | |||||
| Order Code Name | Chromo Leuk/Lymph Rfx CMA | |||||
| Order Loinc | ||||||
| Result Code | 510679 | |||||
| Result Code Name | Cells Counted | |||||
| UofM | ||||||
| Result LOINC | 62361-1 | |||||
| Order Code | 511040 | |||||
| Order Code Name | Chromo Leuk/Lymph Rfx CMA | |||||
| Order Loinc | ||||||
| Result Code | 510680 | |||||
| Result Code Name | Cells Analyzed | |||||
| UofM | ||||||
| Result LOINC | 62360-3 | |||||
| Order Code | 511040 | |||||
| Order Code Name | Chromo Leuk/Lymph Rfx CMA | |||||
| Order Loinc | ||||||
| Result Code | 510681 | |||||
| Result Code Name | Cells Karyotyped | |||||
| UofM | ||||||
| Result LOINC | 55199-4 | |||||
| Order Code | 511040 | |||||
| Order Code Name | Chromo Leuk/Lymph Rfx CMA | |||||
| Order Loinc | ||||||
| Result Code | 510682 | |||||
| Result Code Name | GTG Band Resolution Achieved | |||||
| UofM | ||||||
| Result LOINC | 62358-7 | |||||
| Order Code | 511040 | |||||
| Order Code Name | Chromo Leuk/Lymph Rfx CMA | |||||
| Order Loinc | ||||||
| Result Code | 510683 | |||||
| Result Code Name | Cytogenetic Result | |||||
| UofM | ||||||
| Result LOINC | 62356-1 | |||||
| Order Code | 511040 | |||||
| Order Code Name | Chromo Leuk/Lymph Rfx CMA | |||||
| Order Loinc | ||||||
| Result Code | 511029 | |||||
| Result Code Name | Interpretation | |||||
| UofM | ||||||
| Result LOINC | 62365-2 | |||||
| Order Code | 511040 | |||||
| Order Code Name | Chromo Leuk/Lymph Rfx CMA | |||||
| Order Loinc | ||||||
| Result Code | 511032 | |||||
| Result Code Name | Director Review: | |||||
| UofM | ||||||
| Result LOINC | 48672-0 | |||||
| Order Code | 511040 | |||||
| Order Code Name | Chromo Leuk/Lymph Rfx CMA | |||||
| Order Loinc | ||||||
| Result Code | 052323 | |||||
| Result Code Name | Reflex | |||||
| UofM | ||||||
| Result LOINC | N/A |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 510779 | Reveal (SM) SNP CMA - Oncology | 510795 | Specimen Type | 31208-2 | |
| Reflex 1 | ||||||
| Order Code | 510779 | |||||
| Order Name | Reveal (SM) SNP CMA - Oncology | |||||
| Result Code | 510795 | |||||
| Result Name | Specimen Type | |||||
| UofM | ||||||
| Result LOINC | 31208-2 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 510779 | Reveal (SM) SNP CMA - Oncology | 510796 | # of Genotyping Targets | N/A | |
| Reflex 1 | ||||||
| Order Code | 510779 | |||||
| Order Name | Reveal (SM) SNP CMA - Oncology | |||||
| Result Code | 510796 | |||||
| Result Name | # of Genotyping Targets | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 510779 | Reveal (SM) SNP CMA - Oncology | 510797 | Diagnosis | 62357-9 | |
| Reflex 1 | ||||||
| Order Code | 510779 | |||||
| Order Name | Reveal (SM) SNP CMA - Oncology | |||||
| Result Code | 510797 | |||||
| Result Name | Diagnosis | |||||
| UofM | ||||||
| Result LOINC | 62357-9 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 510779 | Reveal (SM) SNP CMA - Oncology | 510798 | Interpretation | 62365-2 | |
| Reflex 1 | ||||||
| Order Code | 510779 | |||||
| Order Name | Reveal (SM) SNP CMA - Oncology | |||||
| Result Code | 510798 | |||||
| Result Name | Interpretation | |||||
| UofM | ||||||
| Result LOINC | 62365-2 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 510779 | Reveal (SM) SNP CMA - Oncology | 510799 | Director Review: | 48672-0 | |
| Reflex 1 | ||||||
| Order Code | 510779 | |||||
| Order Name | Reveal (SM) SNP CMA - Oncology | |||||
| Result Code | 510799 | |||||
| Result Name | Director Review: | |||||
| UofM | ||||||
| Result LOINC | 48672-0 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 510779 | Reveal (SM) SNP CMA - Oncology | 512123 | 11502-2 | ||
| Reflex 1 | ||||||
| Order Code | 510779 | |||||
| Order Name | Reveal (SM) SNP CMA - Oncology | |||||
| Result Code | 512123 | |||||
| Result Name | ||||||
| UofM | ||||||
| Result LOINC | 11502-2 | |||||