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For hours, walk-ins and appointments.Synonyms
- Polyomavirus BK Quantitation
- Quantitation of BK Virus, DNA, Using Real-time PCR
Expected Turnaround Time
2 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Plasma (EDTA)
Volume
1 mL
Minimum Volume
0.5 mL
Container
Ship in a screw-cap polypropylene frozen transport tube.
Collection
Collect specimen in lavender-top (EDTA) tube. Centrifuge specimen within 24 hours of collection, remove plasma and transfer to a polypropylene screw-capped tube, and freeze. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 48 hours |
Refrigerated | 8 days |
Frozen | 6 months |
Freeze/thaw cycles | Stable x4 |
Causes for Rejection
Quantity not sufficient for analysis; leaking or broken tube; inadequate storage or transport; whole blood; serum
Test Details
Use
Detection and quantitation of BK virus (BKV) in EDTA plasma to be used as an aid in the diagnosis and management of BK virus infections. This assay is traceable to the 1st WHO International Standard for BK Virus for Nucleic Acid Amplification Techniques (NAT)-based assays.
Limitations
The quantitative range is 22 IU/mL-100,000,000 IU/mL. The analytical sensitivity is 22 IU/mL.
Methodology
Real-time polymerase chain reaction (PCR)
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 138962 | BKV Quant PCR | 42587-6 | 139823 | BKV DNA, Quant PCR, Plasma | IU/mL | 42587-6 |
| 138962 | BKV Quant PCR | 42587-6 | 139824 | log10 BKV DNA,Plasma | log10 IU/mL | 48309-9 |
| Reflex Table for BKV DNA, Quant PCR, Plasma | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 139830 | BKV Quant PCR | 139831 | BKV DNA, Quant PCR, Plasma | IU/mL | 42587-6 |
| Reflex Table for BKV DNA, Quant PCR, Plasma | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 139830 | BKV Quant PCR | 139832 | log10 BKV DNA,Plasma | log10 IU/mL | 48309-9 |
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