BCR-ABL1 Oncology, FISH

Test Number 511520

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Synonyms

ALL FISH
CML FISH

Test Details

Methodology

Fluorescence in situ hybridization (FISH)

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Use

This test is used for confirmation/identification of leukemia/lymphoma-related alterations and leukemia/lymphoma monitoring of residual disease.

~~Confirmation/identification of leukemia/lymphoma-related alterations;~~ leukemia/lymphoma monitoring of residual disease.

This test is used for confirmation/identification of leukemia/lymphoma-related alterations and leukemia/lymphoma monitoring of residual disease.

Special Instructions

Include pertinent clinical diagnosis and previous cytogenetic studies on the test request form.

~~Pertinent clinical diagnosis, previous cytogenetic studies, and probe of interest should be included with the specimen. Please provide targeted FISH probe and diagnosis~~.

Include pertinent clinical diagnosis and previous cytogenetic studies on the test request form.

Limitations

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Specimen Requirements

Specimen

Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

~~Fixed-cell pellet from a cytogenetic analysis, slides with metaphases and/or interphase nuclei or bone marrow touch prep slides. Blood, bone marrow, cerebrospinal fluid (CSF)~~.

Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

Volume

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 2 slides with metaphase and/or interphase nuclei or 2 touch preparation slides

~~Fixed-cell pellet, three slides, 10 mL blood (adult), 5 mL blood (pediatric), or 3 mL bone marrow~~

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 2 slides with metaphase and/or interphase nuclei or 2 touch preparation slides

Minimum Volume

1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 1 slide with metaphase and/or interphase nuclei or 1 touch preparation slide (Note: Minimum volumes may not allow for repeat testing.)

~~Fixed-cell pellet from a cytogenetic analysis, two slides, 5 mL blood (adult), 1 mL blood (pediatric), or 1 mL bone marrow~~

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Green-top (~~heparin) tube, pediatric Vacutainer® (optimal), or lavender-top (EDTA) tube~~

Collection Instructions

Utilize sterile technique and Labcorp transport kits when possible.

~~Transport to the testing lab at room temperature; LabCorp transport kit. Do not allow sample to overheat or freez~~e.

Utilize sterile technique and Labcorp transport kits when possible.

Storage Instructions

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

~~Maintain specimen at room temperatur~~e.

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Causes for Rejection

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

Broken ~~or dirty slides; excessive cellular debris or stained slides/paraffin blocks; decalcified bone cores; frozen specimen~~