Test Details
Methodology
Multiplex flow immunoassay
Result Turnaround Time
2 - 3 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Anticentromere B antibodies; anti-dsDNA; antichromatin antibodies; anti-Jo-1; RNP antibodies; antiscleroderma 70 antibodies; Smith antibodies; Sjögren anti-SS-A; Sjögren anti-SS-B
Use
Detect antibodies associated with SLE and mixed connective tissue disease. Anti-Sm is highly specific for SLE. Anti-RNP is found with a variety of rheumatoid diseases. RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1 are extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells.
Special Instructions
This test is intended to be used as a follow-up to Antinuclear Antibodies (ANA) Direct [164855].
Specimen Requirements
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Storage Instructions
Room temperature
Causes for Rejection
Hemolysis; lipemia; gross bacterial contamination; icterus
References
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Eriksson C, Engstrand S, Sundqvist KG, Rantapää-Dahlqvist S. Autoantibody formation in patients with rheumatoid arthritis treated with anti-TNF alpha. Ann Rheum Dis. 2005 Mar; 64(3):403-407. 15297281
Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr. Advanced multiplexed analysis with the FlowMetrix system. Clin Chem. 1997 Sep; 43(9):1749-1756. 9299971
Golightly M, Golightly C. Laboratory diagnosis of autoimmune diseases. MLO Med Lab Obs. 2002 Jul; 34(7):12-16, 20. 12134551
Homburger HA, Cahen YD, Griffiths J, Jacob GL. Detection of antinuclear antibodies: Comparative evaluation of enzyme immunoassay and indirect immunofluorescence methods. Arch Pathol Lab Med. 1998 Nov; 122(11):993-999. 9822128
Isenberg DA. Systemic lupus erythematosus and Sjögren's syndrome: Historical perspective and ongoing concerns. Arthritis Rheum. 2004 Mar; 50(3):681-683. 15022304
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LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 165092 | Antinuclear Ab 9 by Multiplex | 97564-9 | 096343 | Anti-DNA (DS) Ab Qn | IU/mL | 5130-0 |
| 165092 | Antinuclear Ab 9 by Multiplex | 97564-9 | 016354 | RNP Antibodies | AI | 29374-6 |
| 165092 | Antinuclear Ab 9 by Multiplex | 97564-9 | 016362 | Smith Antibodies | AI | 11090-8 |
| 165092 | Antinuclear Ab 9 by Multiplex | 97564-9 | 018707 | Antiscleroderma-70 Antibodies | AI | 27416-7 |
| 165092 | Antinuclear Ab 9 by Multiplex | 97564-9 | 012684 | Sjogren's Anti-SS-A | AI | 17792-3 |
| 165092 | Antinuclear Ab 9 by Multiplex | 97564-9 | 012692 | Sjogren's Anti-SS-B | AI | 17791-5 |
| 165092 | Antinuclear Ab 9 by Multiplex | 97564-9 | 012581 | Antichromatin Antibodies | AI | 51775-5 |
| 165092 | Antinuclear Ab 9 by Multiplex | 97564-9 | 161457 | Anti-Jo-1 | AI | 11565-9 |
| 165092 | Antinuclear Ab 9 by Multiplex | 97564-9 | 164816 | Anti-Centromere B Antibodies | AI | 53982-5 |
| 165092 | Antinuclear Ab 9 by Multiplex | 97564-9 | 164869 | See below: | N/A | |
| Order Code | 165092 | |||||
| Order Code Name | Antinuclear Ab 9 by Multiplex | |||||
| Order Loinc | 97564-9 | |||||
| Result Code | 096343 | |||||
| Result Code Name | Anti-DNA (DS) Ab Qn | |||||
| UofM | IU/mL | |||||
| Result LOINC | 5130-0 | |||||
| Order Code | 165092 | |||||
| Order Code Name | Antinuclear Ab 9 by Multiplex | |||||
| Order Loinc | 97564-9 | |||||
| Result Code | 016354 | |||||
| Result Code Name | RNP Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 29374-6 | |||||
| Order Code | 165092 | |||||
| Order Code Name | Antinuclear Ab 9 by Multiplex | |||||
| Order Loinc | 97564-9 | |||||
| Result Code | 016362 | |||||
| Result Code Name | Smith Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 11090-8 | |||||
| Order Code | 165092 | |||||
| Order Code Name | Antinuclear Ab 9 by Multiplex | |||||
| Order Loinc | 97564-9 | |||||
| Result Code | 018707 | |||||
| Result Code Name | Antiscleroderma-70 Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 27416-7 | |||||
| Order Code | 165092 | |||||
| Order Code Name | Antinuclear Ab 9 by Multiplex | |||||
| Order Loinc | 97564-9 | |||||
| Result Code | 012684 | |||||
| Result Code Name | Sjogren's Anti-SS-A | |||||
| UofM | AI | |||||
| Result LOINC | 17792-3 | |||||
| Order Code | 165092 | |||||
| Order Code Name | Antinuclear Ab 9 by Multiplex | |||||
| Order Loinc | 97564-9 | |||||
| Result Code | 012692 | |||||
| Result Code Name | Sjogren's Anti-SS-B | |||||
| UofM | AI | |||||
| Result LOINC | 17791-5 | |||||
| Order Code | 165092 | |||||
| Order Code Name | Antinuclear Ab 9 by Multiplex | |||||
| Order Loinc | 97564-9 | |||||
| Result Code | 012581 | |||||
| Result Code Name | Antichromatin Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 51775-5 | |||||
| Order Code | 165092 | |||||
| Order Code Name | Antinuclear Ab 9 by Multiplex | |||||
| Order Loinc | 97564-9 | |||||
| Result Code | 161457 | |||||
| Result Code Name | Anti-Jo-1 | |||||
| UofM | AI | |||||
| Result LOINC | 11565-9 | |||||
| Order Code | 165092 | |||||
| Order Code Name | Antinuclear Ab 9 by Multiplex | |||||
| Order Loinc | 97564-9 | |||||
| Result Code | 164816 | |||||
| Result Code Name | Anti-Centromere B Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 53982-5 | |||||
| Order Code | 165092 | |||||
| Order Code Name | Antinuclear Ab 9 by Multiplex | |||||
| Order Loinc | 97564-9 | |||||
| Result Code | 164869 | |||||
| Result Code Name | See below: | |||||
| UofM | ||||||
| Result LOINC | N/A |