Skip to main content

Antiextractable Nuclear Antigens

CPT 86235(x2)
Synonyms
  • Anti-ENA
  • Anti-RNP
  • Anti-Sm

Test Details

Methodology

Multiplex flow immunoassay

Result Turnaround Time

1 - 2 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

Semiquantitative result of IgG class antibodies

Use

Detect antibodies associated with SLE and mixed connective tissue disease. Anti-Sm is highly specific for SLE. Anti-RNP is found with a variety of rheumatoid diseases with high titers associated mainly with MCTD.

Custom Additional Information

Antibodies that produce a speckled ANA pattern are usually directed against extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells. Included in this group of antigens are RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1.

Specimen Requirements

Specimen

Serum

Volume

1 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Reference Range

0.0−0.9 AI

Storage Instructions

Room temperature

Causes for Rejection

Hemolysis; icterus; lipemia; bacterial contamination

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
006338 Antiextractable Nuclear Ag 016354 RNP Antibodies AI 29374-6
006338 Antiextractable Nuclear Ag 016362 Smith Antibodies AI 11090-8
Order Code006338
Order Code NameAntiextractable Nuclear Ag
Order Loinc
Result Code016354
Result Code NameRNP Antibodies
UofMAI
Result LOINC29374-6
Order Code006338
Order Code NameAntiextractable Nuclear Ag
Order Loinc
Result Code016362
Result Code NameSmith Antibodies
UofMAI
Result LOINC11090-8