Test Details
Methodology
Fluorescence in situ hybridization (FISH)
Result Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
This test includes fluorescence in situ hybridization (FISH) for high-grade B-cell lymphoma, targeting rearrangement of BCL2, BCL6 and MYC.
Use
This test is a diagnostic test for non-Hodgkin's lymphoma (NHL) that detects primary genetic changes associated with various types of NHL, including high-grade B-cell lymphoma, follicular lymphoma (FL), Burkitt lymphoma (BL), diffuse large B-cell lymphoma (DLBCL) and those with BCL6 rearrangement.
Special Instructions
Indicate the pertinent clinical diagnosis and previous cytogenetic studies on the test request form. Note: This test number is used for tracking FISH specimens to the laboratory.
Limitations
Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.
This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Molecular mutations |
Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration. |
Specimen Requirements
Specimen
Whole blood, bone marrow, CSF, lymph node, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei, bone marrow touch preparation slides, paraffin slides or block are acceptable for testing.
|
Whole blood, bone marrow, CSF, lymph node, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei, bone marrow touch preparation slides, paraffin slides or block are acceptable for testing. |
Volume
5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 6 slides with metaphase and/or interphase nuclei or 6 touch preparation slides or 6 slides 4- or 5-micron thick with tumor
|
5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 6 slides with metaphase and/or interphase nuclei or 6 touch preparation slides or 6 slides 4- or 5-micron thick with tumor |
Minimum Volume
1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 3 slides with metaphase and/or interphase nuclei or 3 touch preparation slides or 3 slides 4- or 5-micron thick with tumor (Note: Minimum volumes may not allow for repeat testing.)
1 mL |
1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 3 slides with metaphase and/or interphase nuclei or 3 touch preparation slides or 3 slides 4- or 5-micron thick with tumor (Note: Minimum volumes may not allow for repeat testing.) |
Container
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal |
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity. |
Collection Instructions
Utilize sterile technique and Labcorp transport kits when possible.
Utilize sterile technique and Labcorp transport kits when possible. |
Storage Instructions
Transport to the testing laboratory at room temperature, use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.
|
Transport to the testing laboratory at room temperature, use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze. |
Causes for Rejection
Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis
Broken Vacutainer®; broken |
Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis |
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 510344 | High-Grade B-Cell Lymphoma | 510623 | Cells Counted | 62361-1 | ||
| 510344 | High-Grade B-Cell Lymphoma | 510624 | Cells Analyzed | 62360-3 | ||
| 510344 | High-Grade B-Cell Lymphoma | 510625 | FISH Result | 48674-6 | ||
| 510344 | High-Grade B-Cell Lymphoma | 510341 | Interpretation | 50398-7 | ||
| 510344 | High-Grade B-Cell Lymphoma | 510627 | Specimen Type | 31208-2 | ||
| 510344 | High-Grade B-Cell Lymphoma | 510342 | Director Review: | 48672-0 | ||
| Order Code | 510344 | |||||
| Order Code Name | High-Grade B-Cell Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510623 | |||||
| Result Code Name | Cells Counted | |||||
| UofM | ||||||
| Result LOINC | 62361-1 | |||||
| Order Code | 510344 | |||||
| Order Code Name | High-Grade B-Cell Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510624 | |||||
| Result Code Name | Cells Analyzed | |||||
| UofM | ||||||
| Result LOINC | 62360-3 | |||||
| Order Code | 510344 | |||||
| Order Code Name | High-Grade B-Cell Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510625 | |||||
| Result Code Name | FISH Result | |||||
| UofM | ||||||
| Result LOINC | 48674-6 | |||||
| Order Code | 510344 | |||||
| Order Code Name | High-Grade B-Cell Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510341 | |||||
| Result Code Name | Interpretation | |||||
| UofM | ||||||
| Result LOINC | 50398-7 | |||||
| Order Code | 510344 | |||||
| Order Code Name | High-Grade B-Cell Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510627 | |||||
| Result Code Name | Specimen Type | |||||
| UofM | ||||||
| Result LOINC | 31208-2 | |||||
| Order Code | 510344 | |||||
| Order Code Name | High-Grade B-Cell Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510342 | |||||
| Result Code Name | Director Review: | |||||
| UofM | ||||||
| Result LOINC | 48672-0 |