510344: High-Grade B-Cell Lymphoma, FISH

Test Details

Methodology

Fluorescence in situ hybridization (FISH)

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Test Includes

This test includes fluorescence in situ hybridization (FISH) for high-grade B-cell lymphoma, targeting rearrangement of BCL2, BCL6 and MYC.

Use

This test is a diagnostic test for non-Hodgkin's lymphoma (NHL) that detects primary genetic changes associated with various types of NHL, including high-grade B-cell lymphoma, follicular lymphoma (FL), Burkitt lymphoma (BL), diffuse large B-cell lymphoma (DLBCL) and those with BCL6 rearrangement.

Special Instructions

Indicate the pertinent clinical diagnosis and previous cytogenetic studies on the test request form. Note: This test number is used for tracking FISH specimens to the laboratory.

Limitations

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Molecular mutations ~~below the resolution of FISH will not be detected~~.

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Specimen Requirements

Specimen

Whole blood, bone marrow, CSF, lymph node, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei, bone marrow touch preparation slides, paraffin slides or block are acceptable for testing.

~~Blood, bone marrow, slides, paraffin block, lymph node or touch preps~~

Volume

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 6 slides with metaphase and/or interphase nuclei or 6 touch preparation slides or 6 slides 4- or 5-micron thick with tumor

~~7 mL blood (adult), 1-3 mL bone marrow, five slides from cytogenetic analysis or seven slides from paraffin block, one tube fixed pellet, 4 touch prep slides~~

Minimum Volume

1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 3 slides with metaphase and/or interphase nuclei or 3 touch preparation slides or 3 slides 4- or 5-micron thick with tumor (Note: Minimum volumes may not allow for repeat testing.)

1 mL ~~blood, 1 mL bone marrow, and four- to five-micron FFPE slides, 5 touch prep slides~~

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal ~~or lavender-top (EDTA) tube~~

Collection Instructions

Utilize sterile technique and Labcorp transport kits when possible.

Storage Instructions

Transport to the testing laboratory at room temperature, use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

~~Maintain specimens at room temperatur~~e.

Transport to the testing laboratory at room temperature, use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Causes for Rejection

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

Broken Vacutainer®; broken ~~slide~~s