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Adult Acute Lymphoblastic Leukemia (ALL) Profile, FISH

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Synonyms
  • Acute Lymphoblastic Leukemia; Leukemia/Lymphoma-specific FISH

Test Details

Methodology

Fluorescence in situ hybridization (FISH)

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

Fluorescence in situ hybridization (FISH) for acute lymphoblastic leukemia (ALL), targeting rearrangement of BCR/ABL, KMT2A (MLL), CMYC and numerical changes of chromosome 6 and 21q deletion

Use

Diagnostic and prognostic test for acute lymphoblastic leukemia in the pediatric population; detection rate is improved from 50% with a chromosome study to 90% with fluorescence in situ hybridization (FISH)

Special Instructions

Include the pertinent clinical diagnosis and previous cytogenetic studies with the test request form.

Specimen Requirements

Specimen

Blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

Volume

10 mL blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 8 slides with metaphase and/or interphase nuclei or 8 touch preparation slides.

Minimum Volume

5 mL blood (pediatric), 1 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 4 slides with metaphase and/or interphase nuclei or 4 touch preparation slides. (Note: Minimum volumes may not allow for repeat testing.)

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). Adjust tube size to sample volume to avoid heparin toxicity.

Collection Instructions

Transport to the testing laboratory at room temperature, use of a cool pack or a LabCorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Storage Instructions

Maintain the specimen at either room temperature or refrigerate. Do not freeze.

Causes for Rejection

Broken Vacutainer®; broken or stained slides; paraffin blocks; paraffin slides; decalcified bone cores; frozen specimen; quantity not sufficient for analysis

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
511077 FISH Adult ALL Oncology 511059 Specimen Type 31208-2
511077 FISH Adult ALL Oncology 511061 Cells Counted 62361-1
511077 FISH Adult ALL Oncology 511068 Cells Analyzed 62360-3
511077 FISH Adult ALL Oncology 511069 FISH Result 62356-1
511077 FISH Adult ALL Oncology 511073 Interpretation 62365-2
511077 FISH Adult ALL Oncology 511076 Director Review: 48672-0
511077 FISH Adult ALL Oncology 512123 PDF 11502-2
Order Code511077
Order Code NameFISH Adult ALL Oncology
Order Loinc
Result Code511059
Result Code NameSpecimen Type
UofM
Result LOINC31208-2
Order Code511077
Order Code NameFISH Adult ALL Oncology
Order Loinc
Result Code511061
Result Code NameCells Counted
UofM
Result LOINC62361-1
Order Code511077
Order Code NameFISH Adult ALL Oncology
Order Loinc
Result Code511068
Result Code NameCells Analyzed
UofM
Result LOINC62360-3
Order Code511077
Order Code NameFISH Adult ALL Oncology
Order Loinc
Result Code511069
Result Code NameFISH Result
UofM
Result LOINC62356-1
Order Code511077
Order Code NameFISH Adult ALL Oncology
Order Loinc
Result Code511073
Result Code NameInterpretation
UofM
Result LOINC62365-2
Order Code511077
Order Code NameFISH Adult ALL Oncology
Order Loinc
Result Code511076
Result Code NameDirector Review:
UofM
Result LOINC48672-0
Order Code511077
Order Code NameFISH Adult ALL Oncology
Order Loinc
Result Code512123
Result Code NamePDF
UofM
Result LOINC11502-2