Test Details
Methodology
Fluorescence in situ hybridization (FISH)
Result Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Fluorescence in situ hybridization (FISH) for acute lymphoblastic leukemia (ALL), targeting rearrangement of BCR/ABL, KMT2A (MLL), CMYC and numerical changes of chromosome 6 and 21q deletion
Use
Diagnostic and prognostic test for acute lymphoblastic leukemia in the pediatric population; detection rate is improved from 50% with a chromosome study to 90% with fluorescence in situ hybridization (FISH)
Special Instructions
Include the pertinent clinical diagnosis and previous cytogenetic studies with the test request form.
Specimen Requirements
Specimen
Blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.
Volume
10 mL blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 8 slides with metaphase and/or interphase nuclei or 8 touch preparation slides.
Minimum Volume
5 mL blood (pediatric), 1 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 4 slides with metaphase and/or interphase nuclei or 4 touch preparation slides. (Note: Minimum volumes may not allow for repeat testing.)
Container
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). Adjust tube size to sample volume to avoid heparin toxicity.
Collection Instructions
Transport to the testing laboratory at room temperature, use of a cool pack or a LabCorp transport kit is acceptable. Do not allow samples to overheat or freeze.
Storage Instructions
Maintain the specimen at either room temperature or refrigerate. Do not freeze.
Causes for Rejection
Broken Vacutainer®; broken or stained slides; paraffin blocks; paraffin slides; decalcified bone cores; frozen specimen; quantity not sufficient for analysis
LOINC® Map
Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
---|---|---|---|---|---|---|
511077 | FISH Adult ALL Oncology | 511059 | Specimen Type | 31208-2 | ||
511077 | FISH Adult ALL Oncology | 511061 | Cells Counted | 62361-1 | ||
511077 | FISH Adult ALL Oncology | 511068 | Cells Analyzed | 62360-3 | ||
511077 | FISH Adult ALL Oncology | 511069 | FISH Result | 62356-1 | ||
511077 | FISH Adult ALL Oncology | 511073 | Interpretation | 62365-2 | ||
511077 | FISH Adult ALL Oncology | 511076 | Director Review: | 48672-0 | ||
511077 | FISH Adult ALL Oncology | 512123 | 11502-2 | |||
Order Code | 511077 | |||||
Order Code Name | FISH Adult ALL Oncology | |||||
Order Loinc | ||||||
Result Code | 511059 | |||||
Result Code Name | Specimen Type | |||||
UofM | ||||||
Result LOINC | 31208-2 | |||||
Order Code | 511077 | |||||
Order Code Name | FISH Adult ALL Oncology | |||||
Order Loinc | ||||||
Result Code | 511061 | |||||
Result Code Name | Cells Counted | |||||
UofM | ||||||
Result LOINC | 62361-1 | |||||
Order Code | 511077 | |||||
Order Code Name | FISH Adult ALL Oncology | |||||
Order Loinc | ||||||
Result Code | 511068 | |||||
Result Code Name | Cells Analyzed | |||||
UofM | ||||||
Result LOINC | 62360-3 | |||||
Order Code | 511077 | |||||
Order Code Name | FISH Adult ALL Oncology | |||||
Order Loinc | ||||||
Result Code | 511069 | |||||
Result Code Name | FISH Result | |||||
UofM | ||||||
Result LOINC | 62356-1 | |||||
Order Code | 511077 | |||||
Order Code Name | FISH Adult ALL Oncology | |||||
Order Loinc | ||||||
Result Code | 511073 | |||||
Result Code Name | Interpretation | |||||
UofM | ||||||
Result LOINC | 62365-2 | |||||
Order Code | 511077 | |||||
Order Code Name | FISH Adult ALL Oncology | |||||
Order Loinc | ||||||
Result Code | 511076 | |||||
Result Code Name | Director Review: | |||||
UofM | ||||||
Result LOINC | 48672-0 | |||||
Order Code | 511077 | |||||
Order Code Name | FISH Adult ALL Oncology | |||||
Order Loinc | ||||||
Result Code | 512123 | |||||
Result Code Name | ||||||
UofM | ||||||
Result LOINC | 11502-2 |