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Acute Myelocytic Leukemia (AML) Profile, FISH

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  • Updated on 03/31/2025

Test Details

Methodology

Fluorescence in situ hybridization (FISH)

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

FISH for acute myelocytic leukemia (AML), targeting rearrangement of PML-RARA, CBFB, RUNX1/RUNX1T1, KMT2A and numerical changes of 5q, and 7q

Use

This test is a diagnostic and prognostic test for acute myeloid leukemia; detection rate is 80% of the known chromosome causes of AML.

Diagnostic and prognostic test for acute myeloid leukemia; detection rate is 80% of the known chromosome causes of AML.

This test is a diagnostic and prognostic test for acute myeloid leukemia; detection rate is 80% of the known chromosome causes of AML.

Special Instructions

Indicate pertinent clinical diagnosis and previous cytogenetic studies on the test request form.

Limitations

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. 

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Molecular mutations or chromosomal alterations not targeted by the probes included in this profile will not be detected.

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. 

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Specimen Requirements

Specimen

Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

Blood, bone marrow, fixed-cell pellet from a cytogenetic analysis, or slides prepared from a cytogenetic analysis

Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

Volume

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 12 slides with metaphase and/or interphase nuclei or 12 touch preparation slides

10 mL blood (adult), 3 to 5 mL blood (pediatric), 1 to 3 mL bone marrow, one tube fixed-cell pellet, or ten slides

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 12 slides with metaphase and/or interphase nuclei or 12 touch preparation slides

Minimum Volume

1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 6 slides with metaphase and/or interphase nuclei or 6 touch preparation slides (Note: Minimum volumes may not allow for repeat testing.)

1 mL blood (adult/pediatric), 1 mL bone marrow, or six slides

1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 6 slides with metaphase and/or interphase nuclei or 6 touch preparation slides (Note: Minimum volumes may not allow for repeat testing.)

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Green-top (heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Collection Instructions

Utilize sterile technique and Labcorp transport kits when possible.

Utilize sterile technique and Labcorp transport kits when possible.

Storage Instructions

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Maintain specimen at room temperature.

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Causes for Rejection

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

Broken Vacutainer®; broken or dirty slides; excessive cellular debris or stained slides; frozen specimen; decalcified tissue

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
510336 AML FISH Panel 510709 Specimen Type 31208-2
510336 AML FISH Panel 510687 Cells Counted 62361-1
510336 AML FISH Panel 510688 Cells Analyzed 62360-3
510336 AML FISH Panel 510689 FISH Result 48674-6
510336 AML FISH Panel 510328 Interpretation 50398-7
510336 AML FISH Panel 510329 Director Review: 48672-0
510336 AML FISH Panel 512123 PDF 11502-2
Order Code510336
Order Code NameAML FISH Panel
Order Loinc
Result Code510709
Result Code NameSpecimen Type
UofM
Result LOINC31208-2
Order Code510336
Order Code NameAML FISH Panel
Order Loinc
Result Code510687
Result Code NameCells Counted
UofM
Result LOINC62361-1
Order Code510336
Order Code NameAML FISH Panel
Order Loinc
Result Code510688
Result Code NameCells Analyzed
UofM
Result LOINC62360-3
Order Code510336
Order Code NameAML FISH Panel
Order Loinc
Result Code510689
Result Code NameFISH Result
UofM
Result LOINC48674-6
Order Code510336
Order Code NameAML FISH Panel
Order Loinc
Result Code510328
Result Code NameInterpretation
UofM
Result LOINC50398-7
Order Code510336
Order Code NameAML FISH Panel
Order Loinc
Result Code510329
Result Code NameDirector Review:
UofM
Result LOINC48672-0
Order Code510336
Order Code NameAML FISH Panel
Order Loinc
Result Code512123
Result Code NamePDF
UofM
Result LOINC11502-2