Skip to main content

Mycobacterium tuberculosis Susceptibility, Broth Dilution and Confirmation

Test Number 182576
Test number copied
CPT 87188(x5)
Synonyms
  • AFB Susceptibility Testing
  • Susceptibility Testing, M tuberculosis
  • TB Susceptibility Testing

Test Details

Methodology

Broth-based and conventional method.

Result Turnaround Time

30 - 40 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

Use

Determine the susceptibility of Mycobacterium tuberculosis to a profile of antimycobacterial agents.

Special Instructions

Isolates found to be resistant will have the resistance confirmed by the agar proportion method.

Limitations

Susceptibilities cannot be reported if the organism fails to grow in the test medium. Susceptibilities cannot be performed on mixed cultures.

Footnotes

1. Beck-Sague C, Dooley SW, Hutton MD, et al. Hospital outbreak of multidrug-resistant Mycobacterium tuberculosis infections. Factors in transmission to staff and HIV-infected patients.JAMA.1992; 268(10):1280-1286.
2. Dooley SW, Villarino ME, Lawrence M, et al. Nosocomial transmission of tuberculosis in a hospital unit for HIV-infected patients. JAMA. 1992; 267(19):2632-2634.1573751
3. Wolinsky E. Mycobacterial diseases other than tuberculosis. Clin Infect Dis. 1992 Jul; 15(1):1-10.1617048

References

Clinical and Laboratory Standards Institute. Susceptibility Testing of Mycobacteria, Nocardia, and Other Aerobic Actinomycetes; Approved Standard. Document M24-A, vol 23, N°18. Villanova, Pa: CLSI; 2003.
Heifets L. Qualitative and quantitative drug susceptibility tests in mycobacteriology. Am Rev Respir Dis. 1988; 137(5):1217-1222.3195815
Van Scoy RE, Wilkowske CJ. Antituberculous agents.Mayo Clin Proc. 1992; 67(2):179-187.1347579

Custom Additional Information

Susceptibilities should be performed on the first organism isolated from a patient, and at one and three-month intervals if that organism continues to be isolated while the patient is on therapy. Susceptibility tests should be performed in patients with recurrent tuberculosis, as resistant strains are common in recurrent infection. Outbreaks have occurred in health care workers, prison inmates, and prison employees.1,2 Failure to take all drugs in a multidrug regimen can lead to a shift toward resistant organisms and treatment failure. Nontuberculosis Mycobacteria, particularly strains of the M avium complex, are resistant to those drugs used for therapy of M tuberculosis.3

Susceptibilities will be performed based on state regulations and/or statutes. Many states require first isolates of M tuberculosis at an institution to be tested.

Specimen Requirements

Specimen

Mycobacterium tuberculosis isolated from a primary clinical specimen, on a submitted AFB conventional solid medium, and a bacterial swab inoculated with isolated colonies from a pure culture or an AFB broth medium.

Volume

Pure culture isolate on an AFB conventional solid medium and a bacterial swab inoculated with isolated colonies from a pure culture, or a minimum of 1 mL of AFB broth medium.

Container

Conventional or broth medium, tightly sealed, in etiologic agent packaging.

Storage Instructions

Maintain media at room temperature.

Causes for Rejection

Specimen received leaking or in broken transport tube or vial; specimen received in expired transport medium; mixed culture; unlabeled culture or name discrepancy between specimen and test request label

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
182576 Mtb Susceptibility Broth 182575 Organism ID 9825-1
182576 Mtb Susceptibility Broth 998513 Streptomycin 1 ug/mL 48177-0
182576 Mtb Susceptibility Broth 998514 Isoniazid 0.1 ug/mL 25217-1
182576 Mtb Susceptibility Broth 998515 Rifampin 1 ug/mL 25202-3
182576 Mtb Susceptibility Broth 998516 Ethambutol 5 ug/mL 25194-2
182576 Mtb Susceptibility Broth 998517 Pyrazinamide 100 ug/mL 25229-6
182576 Mtb Susceptibility Broth 182577 Agar Proportion Confirmation N/A
Order Code182576
Order Code NameMtb Susceptibility Broth
Order Loinc
Result Code182575
Result Code NameOrganism ID
UofM
Result LOINC9825-1
Order Code182576
Order Code NameMtb Susceptibility Broth
Order Loinc
Result Code998513
Result Code NameStreptomycin 1 ug/mL
UofM
Result LOINC48177-0
Order Code182576
Order Code NameMtb Susceptibility Broth
Order Loinc
Result Code998514
Result Code NameIsoniazid 0.1 ug/mL
UofM
Result LOINC25217-1
Order Code182576
Order Code NameMtb Susceptibility Broth
Order Loinc
Result Code998515
Result Code NameRifampin 1 ug/mL
UofM
Result LOINC25202-3
Order Code182576
Order Code NameMtb Susceptibility Broth
Order Loinc
Result Code998516
Result Code NameEthambutol 5 ug/mL
UofM
Result LOINC25194-2
Order Code182576
Order Code NameMtb Susceptibility Broth
Order Loinc
Result Code998517
Result Code NamePyrazinamide 100 ug/mL
UofM
Result LOINC25229-6
Order Code182576
Order Code NameMtb Susceptibility Broth
Order Loinc
Result Code182577
Result Code NameAgar Proportion Confirmation
UofM
Result LOINCN/A
Reflex Table for Organism ID
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 000000 Antimicrobial Susceptibility 000000 Antimicrobial Susceptibility N/A
Reflex 1
Order Code000000
Order NameAntimicrobial Susceptibility
Result Code000000
Result NameAntimicrobial Susceptibility
UofM
Result LOINCN/A
Reflex Table for Agar Proportion Confirmation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 182691 Mtb AST Confirmation 998522 Streptomycin 2 ug/mL 25205-6
Reflex 1
Order Code182691
Order NameMtb AST Confirmation
Result Code998522
Result NameStreptomycin 2 ug/mL
UofM
Result LOINC25205-6
Reflex Table for Agar Proportion Confirmation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 182691 Mtb AST Confirmation 998523 Streptomycin 10 ug/mL 25206-4
Reflex 1
Order Code182691
Order NameMtb AST Confirmation
Result Code998523
Result NameStreptomycin 10 ug/mL
UofM
Result LOINC25206-4
Reflex Table for Agar Proportion Confirmation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 182691 Mtb AST Confirmation 998519 Isoniazid 0.2 ug/mL 25218-9
Reflex 1
Order Code182691
Order NameMtb AST Confirmation
Result Code998519
Result NameIsoniazid 0.2 ug/mL
UofM
Result LOINC25218-9
Reflex Table for Agar Proportion Confirmation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 182691 Mtb AST Confirmation 998521 Isoniazid 1 ug/mL 25219-7
Reflex 1
Order Code182691
Order NameMtb AST Confirmation
Result Code998521
Result NameIsoniazid 1 ug/mL
UofM
Result LOINC25219-7
Reflex Table for Agar Proportion Confirmation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 182691 Mtb AST Confirmation 998525 Rifampin 1 ug/mL 25202-3
Reflex 1
Order Code182691
Order NameMtb AST Confirmation
Result Code998525
Result NameRifampin 1 ug/mL
UofM
Result LOINC25202-3
Reflex Table for Agar Proportion Confirmation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 182691 Mtb AST Confirmation 998636 Ethambutol 5.0 ug/mL 25194-2
Reflex 1
Order Code182691
Order NameMtb AST Confirmation
Result Code998636
Result NameEthambutol 5.0 ug/mL
UofM
Result LOINC25194-2