The Comparative Analysis of Cytokine Production by a Human 3D Tissue Model Following Exposure to Traditional Cigarette Smoke, Tobacco-Heated Product and E-Cigarette Aerosol

CORESTA Congress 2020 -- The battery of regulatory assays currently used to assess the toxicity of aerosol exposure are limited in their ability to identify changes at the cellular and molecular level. This has prompted a shift towards a more holistic systems biology approach when assessing the effects of exposure to potential toxicants. In this study, we used the V-PLEX human cytokine kit to analyse a panel of 30 disease biomarkers following acute exposure of a human airway 3D tissue model at the ALI using a Vitrocell VC10 to cigarette (3R4F) smoke, tobacco-heated product (THP) and E-cigarette aerosol. Following exposure, post-exposure and recovery (24 hour) medias were collected and biomarker levels quantified. Changes were observed for a number of biomarkers in both the post-exposure and post-recovery media including; IL-1?, IL-8, IL-10, IL12p70, IFN-? and VEGF. Our observations demonstrate the potential of the MSD V-PLEX human cytokine kit in assessing the effects of aerosol